Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees

Imidacloprid (IMD) may affect proteolysis, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and global DNA methylation in honeybees. Queens, drones, and workers aged 1 or 20 days were exposed (free-flying colonies) to IMD (5 ppb and 200 ppb) in their diet...

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Bibliographic Details
Published in:Apidologie 2020-08, Vol.51 (4), p.620-630
Main Authors: Paleolog, Jerzy, Wilde, Jerzy, Siuda, Maciej, Bąk, Beata, Wójcik, Łukasz, Strachecka, Aneta
Format: Article
Language:English
Subjects:
Age
Alanine
Alanine transaminase
Alkaline phosphatase
Animal biology
Apis mellifera
Aspartate aminotransferase
Bees
Biomarkers
Biomedical and Life Sciences
Colonies
Colonies & territories
Deactivation
Deoxyribonucleic acid
DNA
DNA methylation
Drones
Ecotoxicology
Entomology
Hemolymph
Imidacloprid
Insecticides
Life Sciences
Original Article
Population decline
Protease inhibitors
Proteinase inhibitors
Proteolysis
Queens
Senescence
Side effects
Toxicology
Veterinary medicine and animal Health
Workers (insect caste)
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Summary:Imidacloprid (IMD) may affect proteolysis, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and global DNA methylation in honeybees. Queens, drones, and workers aged 1 or 20 days were exposed (free-flying colonies) to IMD (5 ppb and 200 ppb) in their diet. As a result, the colony depopulation did not occurred. IMD disturbed hemolymph/cuticle proteolysis; deactivated most of the cuticle protease inhibitors, activated hemolymph thiol and metal proteases and cuticle thiol proteases; downregulated ALP, ALT, AST; and increased DNA methylation in a caste- and age-dependent manner. The response in queens and workers differed, possibly due to eusocial evolution. Higher IMD dose had greater effects. The responses of ALP, ALT, AST, and DNA may reflect acceleration of biochemical senescence and epigenetic adaptation to IMD. All these biochemical side effects may lead to colony depopulation during future biotic/abiotic stress.
ISSN:0044-8435
1297-9678
DOI:10.1007/s13592-020-00747-4