Ultrafast Light-Driven Electron Transfer in a Ru(II)tris(bipyridine)-Labeled Multiheme Cytochrome

• Published in: Journal of the American Chemical Society 2019-09, Vol.141 (38), p.15190-15200
• Main Authors: van Wonderen, Jessica H, Hall, Christopher R, Jiang, Xiuyun, Adamczyk, Katrin, Carof, Antoine, Heisler, Ismael, Piper, Samuel E. H, Clarke, Thomas A, Watmough, Nicholas J, Sazanovich, Igor V, Towrie, Michael, Meech, Stephen R, Blumberger, Jochen, Butt, Julea N
• Format: Article
• Language: English
• Subjects: Chemical Sciences; or physical chemistry; Theoretical and
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/jacs.9b06858

Multiheme cytochromes attract much attention for their electron transport properties. These proteins conduct electrons across bacterial cell walls and along extracellular filaments and when purified can serve as bionanoelectronic junctions. Thus, it is important and necessary to identify and understand the factors governing electron transfer in this family of proteins. To this end we have used ultrafast transient absorbance spectroscopy, to define heme–heme electron transfer dynamics in the representative multiheme cytochrome STC from Shewanella oneidensis in aqueous solution. STC was photosensitized by site-selective labeling with a Ru­(II)­(bipyridine)3 dye and the dynamics of light-driven electron transfer described by a kinetic model corroborated by molecular dynamics simulation and density functional theory calculations. With the dye attached adjacent to STC Heme IV, a rate constant of 87 × 106 s–1 was resolved for Heme IV → Heme III electron transfer. With the dye attached adjacent to STC Heme I, at the opposite terminus of the tetraheme chain, a rate constant of 125 × 106 s–1 was defined for Heme I → Heme II electron transfer. These rates are an order of magnitude faster than previously computed values for unlabeled STC. The Heme III/IV and I/II pairs exemplify the T-shaped heme packing arrangement, prevalent in multiheme cytochromes, whereby the adjacent porphyrin rings lie at 90° with edge–edge (Fe–Fe) distances of ∼6 (11) Å. The results are significant in demonstrating the opportunities for pump–probe spectroscopies to resolve interheme electron transfer in Ru-labeled multiheme cytochromes.