Reusable surface plasmon resonance biosensor chip for the detection of H1N1 influenza virus

• Published in: Biosensors & bioelectronics 2020-11, Vol.168, p.112561-112561, Article 112561
• Main Authors: Yoo, Haneul, Shin, Junghyun, Sim, Jieun, Cho, Hyunmin, Hong, Seunghun
• Format: Article
• Language: English
• Subjects: Biochemistry, Molecular Biology; Biosensing Techniques; Biosensor; Ferromagnetic pattern; Influenza; Influenza A Virus, H1N1 Subtype; Life Sciences; Magnetic trapping-releasing; Reusable; Surface Plasmon Resonance
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2020.112561

We developed a reusable magnetic surface plasmon resonance (SPR) sensor chip for detecting various target molecules repeatedly in a conventional SPR system. Here, ferromagnetic patterns on a SPR sensor chip were utilized to trap a layer of magnetic particles, and they were utilized as a solid substrate for SPR sensing in a conventional SPR system. After a sensing experiment, the used magnetic particles were removed by external magnetic fields, and a new layer of magnetic particles was immobilized to the SPR sensor chip for additional sensing measurements. Since magnetic particles were trapped on the ferromagnetic patterns, we could use our reusable SPR chip for SPR sensing measurements in a traditional SPR system without any applied magnetic fields. Significantly, ferromagnetic patterns on the sensor chip surface deflected the strong external fields, so that the large aggregation of magnetic particles on the sensor surface was reduced. We demonstrated using a single reusable SPR sensor chip to measure the nucleoprotein (NP) of H1N1 influenza virus solution ranging repeatedly for more than 7 times without significant signal degradation. Also, different target molecules could be repeatedly measured in a single SPR chip. Since our reusable SPR sensor chip can be repeatedly used in a conventional SPR system without any chemical processes for refreshment, the cost for SPR sensing should be significantly reduced. In this case, our reusable SPR sensor chip can be a major breakthrough and can be used for versatile practical applications of SPR sensors. •A reusable surface plasmon resonance (SPR) sensor chip was developed to repeatedly detect target molecules.•Ferromagnetic patterns on solid substrates trapped functionalized magnetic particles, and they were used for SPR sensing.•Our SPR chip can be used in conventional SPR systems without magnetic fields since ferromagnetic patterns hold the particles.•We could repeatedly detect the nucleoprotein of H1N1 influenza virus and other target molecules using a single SPR chip.