Host urokinase-type plasminogen activator participates in the release of malaria merozoites from infected erythrocytes

Malaria infection of red blood cells is associated with plasminogen activation. Surface immunofluorescence and immunoprecipitation experiments, using specific polyclonal and monoclonal antibodies raised against human urokinase, demonstrate that this activity is due to the binding of host urokinase-t...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1997-05, Vol.86 (1), p.49-59
Main Authors: Roggwiller, Emmanuelle, Fricaud, Anne-Catherine, Blisnick, Thierry, Braun-Breton, Catherine
Format: Article
Language:English
Subjects:
Amiloride
Amiloride - pharmacology
Animals
Erythrocyte Membrane
Erythrocyte Membrane - parasitology
Erythrocytes
Erythrocytes - enzymology
Erythrocytes - parasitology
Fluorescent Antibody Technique
Humans
Immunoglobulin G
Invasion
Isoflurophate
Isoflurophate - pharmacology
Life Sciences
Malaria
Malaria, Falciparum
Malaria, Falciparum - blood
Malaria, Falciparum - enzymology
Merozoite release
Mice
Microbiology and Parasitology
Plasminogen activator
Plasmodium falciparum
Plasmodium falciparum - pathogenicity
Plasmodium falciparum - physiology
Urokinase-Type Plasminogen Activator
Urokinase-Type Plasminogen Activator - blood
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Summary:Malaria infection of red blood cells is associated with plasminogen activation. Surface immunofluorescence and immunoprecipitation experiments, using specific polyclonal and monoclonal antibodies raised against human urokinase, demonstrate that this activity is due to the binding of host urokinase-type plasminogen activator to the surface of erythrocytes infected by mature forms of Plasmodium falciparum malaria parasites. Depletion of urokinase from the culture medium leads to the inhibition of merozoite release and the accumulation of segmenter-infected erythrocytes; this inhibition is reversed by the addition of human single-chain or two-chain urokinase. These findings are consistent with host urokinase being involved in the process of merozoite release from the red blood cell.
ISSN:0166-6851
1872-9428
DOI:10.1016/S0166-6851(97)02848-X