Plant mitochondria import DNA via alternative membrane complexes involving various VDAC isoforms

•Plant organelles import DNA of different size classes through distinct channels.•DNA import depends on both the size and molarity of the imported substrate.•VDAC isoforms show differential involvement in the formation of alternative channels.•We propose several membrane proteins as VDAC partners in...

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Bibliographic Details
Published in:Mitochondrion 2021-09, Vol.60, p.43-58
Main Authors: Tarasenko, Tatiana A., Klimenko, Ekaterina S., Tarasenko, Vladislav I., Koulintchenko, Milana V., Dietrich, André, Weber-Lotfi, Frédérique, Konstantinov, Yuri M.
Format: Article
Language:English
Subjects:
Adenine nucleotide translocase
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis thaliana
Biological Transport - genetics
DNA import
DNA, Mitochondrial - genetics
DNA, Plant - genetics
Gene Deletion
Life Sciences
Mitochondria - genetics
Mitochondrial Membranes - physiology
Plant mitochondria
Protoplasts
Solanum tuberosum
Solanum tuberosum - genetics
Solanum tuberosum - metabolism
Vegetal Biology
Voltage-dependent anion channel isoforms
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Summary:•Plant organelles import DNA of different size classes through distinct channels.•DNA import depends on both the size and molarity of the imported substrate.•VDAC isoforms show differential involvement in the formation of alternative channels.•We propose several membrane proteins as VDAC partners in these pathways. Mitochondria possess transport mechanisms for import of RNA and DNA. Based on import into isolated Solanum tuberosum mitochondria in the presence of competitors, inhibitors or effectors, we show that DNA fragments of different size classes are taken up into plant organelles through distinct channels. Alternative channels can also be activated according to the amount of DNA substrate of a given size class. Analyses of Arabidopsis thaliana knockout lines pointed out a differential involvement of individual voltage-dependent anion channel (VDAC) isoforms in the formation of alternative channels. We propose several outer and inner membrane proteins as VDAC partners in these pathways.
ISSN:1567-7249
1872-8278
DOI:10.1016/j.mito.2021.07.006