Metabolism regulation during salt exposure in the halophyte Cakile maritima

• Published in: Environmental and experimental botany 2020-09, Vol.177, p.104075-14, Article 104075
• Main Authors: Arbelet-Bonnin, Delphine, Blasselle, Camille, Rose Palm, Emily, Redwan, Mirvat, Ponnaiah, Maharajah, Laurenti, Patrick, Meimoun, Patrice, Gilard, Françoise, Gakière, Bertrand, Mancuso, Stefano, El-Maarouf-Bouteau, Hayat, Bouteau, François
• Format: Article
• Language: English
• Subjects: Botanics; Cakile maritima; Environmental Sciences; Halophytes; Life Sciences; Metabolome; Salt stress; Vegetal Biology
• ISSN: 0098-8472; 1873-7307
• DOI: 10.1016/j.envexpbot.2020.104075

•The osmoprotection of Cakile maritima in saline stress is due solely to the accumulation of amino acids.•A Cakile maritima leaves metabolome was managed over time and in three salt conditions (0 mM, 100 mM and 400 mM NaCl).•Photosynthetic and metabolome analysis reveal the facultativ halophily of Cakile maritima in moderate saline condition . The halophyte Cakile maritima is a Brassicacea that has developed numerous mechanisms for managing salt. In the present study, we analyze the metabolic responses of C. maritima to increasing salt exposure in parallel with growth and photosynthetic parameters. At 10 days, 100 mM NaCl treatment has no effect, whereas 400 mM treatment decreases both growth and photosynthetic capacity. Accordingly, the metabolism was weakly impacted at 100 mM NaCl with an increase in only a few amino acids and sugars, whereas 400 mM treated plants shows noticeable changes: an increase in amino acid abundance, sugars decrease and an organic acid depletion. At 20 days, 400 mM treatment leads to more severe effects on growth and photosynthesis, whereas plant growth remains unaffected by the 100 mM NaCl treatment, despite a reduction in photosynthetic capacity. Plants treated with 400 mM NaCl present an amplified metabolic response with additional metabolites reflecting salt stress as GABA, proline and glycine. One noticeable feature of halophily in C. maritima is the increase in sugar content at low stress whereas longer or higher stress lead to a decrease in sugar content. In high salt conditions the stimulation of amino acid biosynthesis is the main strategy for osmoprotection to cope for salt stress.