Influence of sensory neuropeptides on human cutaneous wound healing process

Abstract Background Close interactions exist between primary sensory neurons of the peripheral nervous system (PNS) and skin cells. The PNS may be implicated in the modulation of different skin functions as wound healing. Objective Study the influence of sensory neurons in human cutaneous wound heal...

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Bibliographic Details
Published in:Journal of dermatological science 2014-06, Vol.74 (3), p.193-203
Main Authors: Chéret, J, Lebonvallet, N, Buhé, V, Carre, J.L, Misery, L, Le Gall-Ianotto, C
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion
Cell Differentiation
Cell Proliferation
Coculture Techniques
Collagen - metabolism
Cutaneous wound healing
Dermatology
Humans
In Vitro Techniques
Life Sciences
Matrix Metalloproteinases - metabolism
Myofibroblasts - physiology
Neuropeptides
Neuropeptides - physiology
Rats
Re-Epithelialization
Sensory neurons
Sensory Receptor Cells - physiology
Skin - cytology
Wound Healing
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Summary:Abstract Background Close interactions exist between primary sensory neurons of the peripheral nervous system (PNS) and skin cells. The PNS may be implicated in the modulation of different skin functions as wound healing. Objective Study the influence of sensory neurons in human cutaneous wound healing. Methods We incubated injured human skin explants either with rat primary sensory neurons from dorsal root ganglia (DRG) or different neuropeptides (vasoactive intestinal peptide or VIP, calcitonin gene-related peptide or CGRP, substance P or SP) at various concentrations. Then we evaluated their effects on the proliferative and extracellular matrix (ECM) remodeling phases, dermal fibroblasts adhesion and differentiation into myofibroblasts. Results Thus, DRG and all studied neuromediators increased fibroblasts and keratinocytes proliferation and act on the expression ratio between collagen type I and type III in favor of collagen I, particularly between the 3rd and 7th day of culture. Furthermore, the enzymatic activities of matrix metalloprotesases (MMP-2 and MMP-9) were increased in the first days of wound healing process. Finally, the adhesion of human dermal fibroblasts and their differentiation into myofibroblasts were promoted after incubation with neuromediators. Interestingly, the most potent concentrations for each tested molecules, were the lowest concentrations, corresponding to physiological concentrations. Conclusion Sensory neurons and their derived-neuropeptides are able to promote skin wound healing.
ISSN:0923-1811
1873-569X
DOI:10.1016/j.jdermsci.2014.02.001