Determination of new types of DNA lesions in human sperm

Careful attention has been focused recently on DNA quality in human IVF. Therefore a variety of methods has been developed to evaluate DNA integrity, especially concerning fragmentation. Using liquid chromatography and mass spectrometry (LC/MS/MS) for our best sperm samples, we have established refe...

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Bibliographic Details
Published in:Zygote (Cambridge) 2008-02, Vol.16 (1), p.9-13
Main Authors: Badouard, C., Ménézo, Y., Panteix, G., Ravanat, J.L., Douki, T., Cadet, J., Favier, A.
Format: Article
Language:English
Subjects:
Adenosine - analogs & derivatives
Adenosine - metabolism
Chemical Sciences
Chromatography, High Pressure Liquid
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - metabolism
DNA Adducts - metabolism
DNA Damage
DNA lesions
Guanosine - analogs & derivatives
Guanosine - metabolism
HPLC – MS/MS
Humans
Lipid peroxidation
Male
Mass Spectrometry
Organic chemistry
Oxidative Stress
Sperm
Spermatozoa - metabolism
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Summary:Careful attention has been focused recently on DNA quality in human IVF. Therefore a variety of methods has been developed to evaluate DNA integrity, especially concerning fragmentation. Using liquid chromatography and mass spectrometry (LC/MS/MS) for our best sperm samples, we have established reference values for several oxidative lesions, in order to gain insights into the cause of DNA lesions. Besides 8-oxodeoxyguanosine, we found rather high levels of two ethenonucleosides: 1,N6-ethenoadenosine and 1,N2-ethenoguanosine. These compounds probably arise from a reaction with 4-hydroxy-2-nonenal, the main aldehyde compound released during lipid peroxidation, or after occupational exposure to vinyl chloride. The quantity of chlorinated bases detected is low. All of this decay has to be repaired by the oocytes at the time of fertilization or immediately after. This aspect should not be overlooked in assisted reproductive technology, in order to understand risks and limitations.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199407004340