Complete genome sequence of Ostreid herpesvirus type 1 µVar isolated during mortality events in the Pacific oyster Crassostrea gigas in France and Ireland

Infections with Ostreid herpesvirus 1 (OsHV-1) microvariants in young Pacific oysters are associated with massive mortality events and significant economic losses. Previous studies, focusing on few regions of the genome, have revealed the genomic diversity of these genotypes with respect to the refe...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2017-09, Vol.509, p.239-251
Main Authors: Burioli, E.A.V., Prearo, M., Houssin, M.
Format: Article
Language:English
Subjects:
Animals
Cluster Analysis
Complete genome
Crassostrea - virology
Crassostrea gigas
DNA, Viral - chemistry
DNA, Viral - genetics
France
Gene Order
Genome, Viral
Herpesviridae - genetics
Herpesviridae - isolation & purification
INDEL Mutation
Indels
Ireland
Life Sciences
NGS
Ostreid herpesvirus-1 µVar
Phylogeny
Sequence Analysis, DNA
Sequence Homology
Synteny
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Summary:Infections with Ostreid herpesvirus 1 (OsHV-1) microvariants in young Pacific oysters are associated with massive mortality events and significant economic losses. Previous studies, focusing on few regions of the genome, have revealed the genomic diversity of these genotypes with respect to the reference type. We used a NGS process to sequence the whole genome of the OsHV-1 µVar in infected individuals, collected during mortality events in France and Ireland. The final genome length of OsHV-1 µVar was approximately 205kbp, shorter than the reference genotype and the overall genome organisation resembled herpes simplex viruses. 94.4% similarity was observed with the OsHV-1 reference genotype. Large indels, including five deletions and three insertions were found to induce the loss and the addition of several ORFs, summed with codon substitutions in 64% of genes shared with the reference type. This diversity raises the question of the exact origin and evolution of OsHV-1 µVar. [Display omitted] •Complete genome sequencing of OsHV-1 µVar was performed using an NGS process.•Final genome length of OsHV-1 µVar was approximately 205kbp.•94.4% similarity was observed with the OsHV-1 reference genotype.•Codon substitutions in 64% of genes shared with reference type and 8 large indels.•OsHV-1 reference and µVar genotypes may have separated long ago.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2017.06.027