Microwave Atmospheric Plasma: A Versatile and Fast Way to Confer Antimicrobial Activity toward Direct Chitosan Immobilization onto Poly(lactic acid) Substrate

• Published in: ACS applied bio materials 2021-10, Vol.4 (10), p.7445-7455
• Main Authors: Carette, Xavier, Mincheva, Rosica, Herbin, Morgane, Cabecas Segura, Paloma, Wattiez, Ruddy, Noirfalise, Xavier, Thai, Cuong, Leclere, Philippe, Godfroid, Thomas, Boudifa, Mohamed, Kerdjoudj, Halima, Jolois, Olivier, Raquez, Jean-Marie
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - pharmacology; Chitosan - chemistry; Life Sciences; Microwaves; Polyesters
• ISSN: 2576-6422; 2576-6422
• DOI: 10.1021/acsabm.1c00206

In this study, a simple method to immobilize chitosan on a poly­(lactic acid) (PLA) surface was developed in a fast manner. The immobilization was realized in two steps. First, an atmospheric plasma (MWAP) torch was used to modify the PLA surface in less than 5 min in order to create enough activated sites toward the chitosan adhesion, followed by a direct dip coating to spread and immobilize chitosan on this MWAP-modified PLA surface. The modification of the PLA surface properties was confirmed by X-ray photoelectron spectroscopy (XPS), water contact angle, and atomic force microscopy. It resulted that the activated species derived from the plasma torch, i.e., hydroxyl and carboxylic acid moieties, enabled an increase of the hydrophilicity of the PLA surface. Interestingly, this activated surface allows a good spreading of chitosan solution from dip coating and leads to a homogeneous stable coating. Our XPS results bring us the hypothesis that the stabilization of the chitosan layer is mainly induced by noncovalent interactions such as hydrogen bonding and electrostatic interactions. A first insight into the biological properties of theses surfaces was assessed in terms of the antimicrobial activity of the here-designed surfaces.