Cloning and characterization of a gene encoding a malic enzyme involved in anaerobic growth in Mucor circinelloides

A 3193 bp contiguous sequence has been cloned from the oleaginous fungus Mucor circinelloides, that contains a full-length gene encoding a putative NADP+[ratio ]dependent malic enzyme (EC. 1.1.1.40). The cloned DNA contains a 2154 bp putative open reading frame containing five introns and encoding a...

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Bibliographic Details
Published in:Mycological research 2005-04, Vol.109 (4), p.461-468
Main Authors: LI, Yonghua, ADAMS, Ian P., WYNN, James P., RATLEDGE, Colin
Format: Article
Language:English
Subjects:
amino acid sequences
Anaerobiosis
Cloning, Molecular
complementary DNA
exons
introns
Isoenzymes - isolation & purification
Isoenzymes - metabolism
Life Sciences
Lipid Metabolism
Malate Dehydrogenase - genetics
Malate Dehydrogenase - isolation & purification
Malate Dehydrogenase - metabolism
malic enzyme
molecular sequence data
Mucor
Mucor - enzymology
Mucor - genetics
Mucor - growth & development
Mucor - metabolism
Mucor circinelloides
nucleotide sequences
Open Reading Frames
promoter regions
Saccharomyces cerevisiae
sequence analysis
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Summary:A 3193 bp contiguous sequence has been cloned from the oleaginous fungus Mucor circinelloides, that contains a full-length gene encoding a putative NADP+[ratio ]dependent malic enzyme (EC. 1.1.1.40). The cloned DNA contains a 2154 bp putative open reading frame containing five introns and encoding a protein of 616 amino acids. The gene encoded what appeared to be an anaerobic isoform of malic enzyme (isoform II); this conclusion is supported by transcript analysis and by the fact that the ORF contains an N-terminal mitochondrial target sequence (a similar cellular location was identified for the anaerobic malic enzyme in Saccharomyces cerevisiae; Boles et al. 1998). The cloned gene did not encode either isoform III (the isoform associated with active growth) or isoform IV (associated with lipid accumulation) previously identified. M. circinelloides therefore must possess (at least) two structural genes for malic enzyme.
ISSN:0953-7562
1469-8102
DOI:10.1017/S0953756205002480