Structure–Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System

Anisotropic environments can drastically alter the spectroscopy and photochemistry of molecules, leading to complex structure‐function relationships. We examined this using fluorescent proteins as easy‐to‐modify model systems. Starting from a single scaffold, we have developed a range of 27 photochr...

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Bibliographic Details
Published in:Angewandte Chemie 2021-04, Vol.60 (18), p.10073-10081
Main Authors: De Zitter, Elke, Hugelier, Siewert, Duwé, Sam, Vandenberg, Wim, Tebo, Alison G., Van Meervelt, Luc, Dedecker, Peter
Format: Article
Language:English
Subjects:
Biochemistry, Molecular Biology
Biophysics
Crystal structure
Datasets
Environmental effects
Fluorescence
fluorescent proteins
Hydrogen bonding
Life Sciences
Modulators
Molecular structure
Photochemistry
photochromism
Polarity
Principal components analysis
Protein engineering
Proteins
Spectroscopy
Spectrum analysis
Structural Biology
Structure-function relationships
Water chemistry
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Summary:Anisotropic environments can drastically alter the spectroscopy and photochemistry of molecules, leading to complex structure‐function relationships. We examined this using fluorescent proteins as easy‐to‐modify model systems. Starting from a single scaffold, we have developed a range of 27 photochromic fluorescent proteins that cover a broad range of spectroscopic properties, including the determination of 43 crystal structures. Correlation and principal component analysis confirmed the complex relationship between structure and spectroscopy, but also allowed us to identify consistent trends and to relate these to the spatial organization. We find that changes in spectroscopic properties can come about through multiple underlying mechanisms, of which polarity, hydrogen bonding and presence of water molecules are key modulators. We anticipate that our findings and rich structure/spectroscopy dataset can open opportunities for the development and evaluation of new and existing protein engineering methods. Anisotropic environments can drastically alter the spectroscopy and photochemistry of molecules. A large structural and spectroscopic dataset of closely‐related protein variants is used to study how the anisotropic chromophore environment of a fluorescent protein determines its properties.
ISSN:1433-7851
0044-8249
1521-3773
1521-3757
DOI:10.1002/anie.202015201