Calf primary hepatocyte culture as a tool for anabolic steroid metabolism studies

Calf hepatocyte cultures were developed for the predictive analysis of in vivo xenobiotic biotransformations. The goal of this work was to show the feasibility of a system based on calf primary hepatocyte cultures using radiolabelled molecules to study xenobiotic metabolism rather than to study exha...

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Bibliographic Details
Published in:Analyst (London) 1998-12, Vol.123 (12), p.2489-2492
Main Authors: CLOUET, A.-S, LE BIZEC, B, BOERLEN, F, MONTEAU, F, ANDRE, F
Format: Article
Language:English
Subjects:
Anabolic Agents
Anabolic Agents - metabolism
Animal productions
Animals
Biological and medical sciences
Cattle
Cattle - metabolism
Cells, Cultured
Chemical Sciences
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Life Sciences
Liver
Liver - metabolism
Male
Nandrolone
Nandrolone - analogs & derivatives
Nandrolone - metabolism
Nandrolone Decanoate
Santé publique et épidémiologie
Terrestrial animal productions
Vertebrates
Xenobiotics
Xenobiotics - metabolism
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Summary:Calf hepatocyte cultures were developed for the predictive analysis of in vivo xenobiotic biotransformations. The goal of this work was to show the feasibility of a system based on calf primary hepatocyte cultures using radiolabelled molecules to study xenobiotic metabolism rather than to study exhaustively the metabolism of a particular steroid. 19-Nortestosterone was chosen as a model substrate because of its relatively well known metabolism in the bovine species. Incubation of a mixture of tritiated and non-tritiated 19-nortestosterone was investigated in cell culture in order to target the biosynthesized metabolites. After extraction and purification, some of the in vitro metabolite structures were elucidated by GC-MS and compared with the main urinary metabolites detected in vivo after intramuscular injection of 19-nortestosterone into a calf. 19-Norepitestosterone, the main in vivo metabolite, was identified in vitro. However, the main in vitro metabolites were mostly in an oxidized form (4-estrene-3,17-dione, hydroxy-4-estrene-3,17-dione).
ISSN:0003-2654
1364-5528
DOI:10.1039/a805216f