Sustained delivery of siRNA/mesoporous silica nanoparticle complexes from nanofiber scaffolds for long-term gene silencing

[Display omitted] A low toxicity and efficient delivery system is needed to deliver small interfering RNAs (siRNA) in vitro and in vivo. The use of mesoporous silica nanoparticles (MSN) is becoming increasingly common due to its biocompatibility, tunable pore size and customizable properties. Howeve...

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Bibliographic Details
Published in:Acta biomaterialia 2018-08, Vol.76, p.164-177
Main Authors: Pinese, Coline, Lin, Junquan, Milbreta, Ulla, Li, Mingqiang, Wang, Yucai, Leong, Kam W., Chew, Sing Yian
Format: Article
Language:English
Subjects:
Adsorption
Biocompatibility
Cancer
Collagen
Collagen (type I)
Controlled release
Electrospinning
Encapsulation
Feasibility studies
Fibers
Foreign body reaction
Gene silencing
Host-implant integration
Implantation
In vivo methods and tests
Life Sciences
miRNA
Nanofibers
Nanoparticles
Pore size
Porosity
Proteins
Scaffolds
Silica
Silicon dioxide
siRNA
Surface chemistry
Surgical implants
Sustained release
Tissue engineering
Toxicity
Transfection
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Summary:[Display omitted] A low toxicity and efficient delivery system is needed to deliver small interfering RNAs (siRNA) in vitro and in vivo. The use of mesoporous silica nanoparticles (MSN) is becoming increasingly common due to its biocompatibility, tunable pore size and customizable properties. However, bolus delivery of siRNA/MSN complexes remains suboptimal, especially when a sustained and long-term administration is required. Here, we utilized electrospun scaffolds for sustained delivery of siRNA/MSN-PEI through surface adsorption and nanofiber encapsulation. As a proof-of-concept, we targeted collagen type I expression to modulate fibrous capsule formation. Surface adsorption of siRNA/MSN-PEI provided sustained availability of siRNA for at least 30 days in vitro. As compared to conventional bolus delivery, such scaffold-mediated transfection provided more effective gene silencing (p 
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2018.05.054