A new fluorescence probe for detection of Cu+2 in blood samples: Circuit logic gate

• Published in: Analytical biochemistry 2022-02, Vol.639 (1), p.114525-114525, Article 114525
• Main Authors: Talesh Ramezani, Ali, Rabiei, Razieh, Badiei, Alireza, Mohammadi Ziarani, Ghodsi, Ghasemi, Jahan B.
• Format: Article
• Language: English
• Subjects: Cations, Divalent - analysis; Cations, Divalent - blood; Chemical Sciences; Chitosan; Chitosan - analogs & derivatives; Copper - analysis; Copper - blood; Copper ion detection; Fluorescent Dyes - chemistry; Humans; Organic chemistry; Oxyquinoline - chemistry; Spectrometry, Fluorescence - methods; Turn on-off fluorescence probe; Water - analysis; Wilson's disease
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2021.114525

A Fluorescence probe was designed based on 8-hydroxyquinoline chitosan silica precursor (HQCS) for selective detection of Al3+, Cu2+. The HQCS has no observable fluorescence signal, but after the addition of Al3+, a huge fluorescence signal appeared, and the selective quenching was absorbed after the addition of Cu2+. The effect of other different cations, including Cu2+, Mg2+, Ca2+, Pb2+, Zn2+, Hg2+, Ag+, Fe3+, and K+ was studied. The addition of Cu2+ to the probe (HQCSAL) decreased the fluorescence very repeatable, and the variation of the fluorescence vs. Cu2+ was monotonic and linear. Therefore, the prepared probe was used to determine Cu2+ ions in real samples. The mechanism of fluorescence variation by adding cations to the probe solution was studied using the Stern-Volmer equation. Under the optimum conditions, the linear range and detection limit were 3.5–31 μM and 1 μM, respectively. The probe accuracy on the copper determination in the blood and tap waters was comparable to the ICP-OES results. The circuit logic gate mimic was designed for the fluorescence behavior of the probe constituents. [Display omitted] •A facile fluorescence probe was synthesized based on 8-hydroxyquinoline chitosan silica (HQCS).•HQCS showed a selective turn on-off fluorescence behavior towards aluminum and copper ions.•The probe showed a repeatable and a linear response towards aluminum and copper ions.•The static fluorescence quenching mechanism was proved by absorption spectra.•Copper ions was successfully determined in tap-water and blood samples.