Dynamics of epigenetic modifications in ICSI embryos from in vitro‐produced spermatozoa

• Published in: Andrology (Oxford) 2021-03, Vol.9 (2), p.640-656
• Main Authors: Oblette, Antoine, Rives‐Feraille, Aurélie, Dumont, Ludovic, Delessard, Marion, Saulnier, Justine, Rives, Nathalie, Rondanino, Christine
• Format: Article
• Language: English
• Subjects: Deoxyribonucleic acid; DNA; DNA methylation; Embryos; epigenetic modifications; Epigenetics; in vitro spermatogenesis; intracytoplasmic sperm injection; Life Sciences; mouse model; prepubertal testis; pre‐implantation embryo development; Reproductive Biology; Sexual reproduction
• ISSN: 2047-2919; 2047-2927; 2047-2927
• DOI: 10.1111/andr.12926

Background In prepubertal boys with cancer, fertility preservation relies on testicular tissue freezing before treatment. In vitro maturation of frozen/thawed tissues could be one of the procedures envisaged to restore the fertility of cured patients. It is necessary to ascertain in the mouse model that in vitro‐generated spermatozoa are able to ensure embryo development, without altering the epigenetic processes occurring during the pre‐implantation period. Objectives The aims of the present study were to investigate the fertilizing ability of in vitro‐produced spermatozoa and explore several epigenetic marks at different stages of embryo development. Materials and methods Fresh or controlled slow‐frozen (CSF)/thawed testicular tissues from 6 to 7 days post‐partum (dpp) mice were cultured for 30 days. Intracytoplasmic sperm injection (ICSI) experiments were performed using in vitro‐produced spermatozoa. Testicular spermatozoa from 36 to 37 dpp mice were used as in vivo controls. DNA methylation/hydroxymethylation and histone post‐translational modifications (H3K4me3, H3K27me3 and H3K9ac) were analysed by immunofluorescence from the zygote to the blastocyst stages. Results The spermatozoa generated in cultures of fresh or CSF testicular tissues were able to initiate embryonic development. The freezing of prepubertal testicular tissues limits the production of spermatozoa in vitro and the fertilization rate after ICSI. Similar levels of H3K4me3, H3K27me3 and H3K9ac were found in ICSI embryos derived from in vitro‐ and in vivo‐produced spermatozoa. DNA methylation levels were increased in 4‐cell embryos and morula obtained by ICSI with in vitro‐produced spermatozoa. Discussion and conclusion Our study shows for the first time that the use of in vitro‐produced spermatozoa alters DNA methylation/demethylation dynamics but has little impact on H3K4me3, H3K27me3 and H3K9ac levels in mouse early embryos. Further work will have to be performed to determine whether the use of these gametes is not deleterious for embryo development before considering a human application.