Development of support based on chitosan and cellulose nanocrystals for the immobilization of anti-Shiga toxin 2B antibody

•Membrane based on chitosan, cellulose nanocrystals, and glycerol was developed.•Membrane was optimized for anti-Shiga toxin 2B antibody immobilization.•Cellulose nanocrystals (0.6 %) & chitosan (95/2500) enhanced immobilization efficiency.•Antibody immobilization optimized by fractional factori...

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Bibliographic Details
Published in:Carbohydrate polymers 2020-03, Vol.232, p.115785-115785, Article 115785
Main Authors: Shankar, Shiv, Baraketi, Amina, D’Auria, Sabato, Fraschini, Carole, Salmieri, Stephane, Jamshidian, Majid, Etty, Marie Christine, Lacroix, Monique
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Antibody immobilization
Antigen-Antibody Reactions
Box-Behnken design
Cellulose
Cellulose nanocrystals
Chitosan
Fractional factorial design
Life Sciences
Nanostructures
Shiga Toxin
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Summary:•Membrane based on chitosan, cellulose nanocrystals, and glycerol was developed.•Membrane was optimized for anti-Shiga toxin 2B antibody immobilization.•Cellulose nanocrystals (0.6 %) & chitosan (95/2500) enhanced immobilization efficiency.•Antibody immobilization optimized by fractional factorial and Box-Behnken designs.•Membrane has detection limit of log 1 CFU/mL Escherichia coli O157:H7. This work describes the development of membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY), and optimization of the formulation for immobilization of monoclonal anti-Shiga toxin 2B antibody (mAnti-stx2B-Ab) for E. coli O157:H7 detection. The effect of CHI deacetylation degree & viscosity, CNCs and GLY concentrations on Anti-stx2B-Ab immobilization efficiency was evaluated. Fractional factorial and Box-Behnken designs were applied to screen the effects of compounds interactions and optimize their concentrations for detection of Anti-stx2B-Ab. The results demonstrated that the use of 0.6 % (w/v) CNCs improved significantly the Anti-stx2B-Ab immobilization and the level of signal detection. The detection limit of Escherichia coli O157:H7 by developed optimized membrane is 1 log CFU/mL. The time needed for detection of E. coli O157:H7 was only 4 h of enrichment compared to 24 h with conventional methods. The developed immobilization support has potential for future pathogen detection in food and biomedical samples.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2019.115785