Kinetic analysis of a Saccharomyces cerevisiae strain adapted for improved growth on glycerol: Implications for the development of yeast bioprocesses on glycerol

• Published in: Bioresource technology 2011, Vol.102 (2), p.1521-1527
• Main Authors: Ochoa-Estopier, A., Lesage, J., Gorret, N., Guillouet, S.E.
• Format: Article
• Language: English
• Subjects: Adaptation; Adaptation, Physiological - drug effects; Aerobiosis - drug effects; Biodiesel; Biological and medical sciences; Biomass; Biotechnology; Carbon; Carbon - pharmacology; Chemical and Process Engineering; Cultivation; Engineering Sciences; Ethyl alcohol; Feedstock; Fermentation; Fermentation - drug effects; Food engineering; Fundamental and applied biological sciences. Psychology; Glycerol; Glycerol - pharmacology; Glycerols; Kinetics; Life Sciences; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Oxidation-Reduction - drug effects; Oxygen - pharmacology; Residues; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Strain; Time Factors
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2010.08.003

Glycerol is an agro-industrial residue generated in high amounts during the biodiesel production. The growing production of biodiesel is creating a worldwide glycerol surplus. Therefore, replacing sugar-based feedstock in bioprocesses by glycerol could be potentially attractive. Saccharomyces cerevisiae is one of the most commonly used microorganisms in the agri-food industry and therefore currently produced in large quantities from sugar-based feedstock. Unfortunately, growth of S. cerevisiae strains on glycerol is very low with reported μ max around 0.01 h −1. This study demonstrates that successive growth of the S. cerevisiae CBS 8066, CEN.PK 113-7D and Ethanol Red on glycerol as sole carbon source considerably improved the μ max from 0.01 up to 0.2 h −1. The “adapted strain” CBS 8066-FL20 was kinetically characterized during aerobic and oxygen-limited cultivation in bioreactor and the results discussed in terms of their implication for developing glycerol-based S. cerevisiae bioprocesses.