Interactions of the ribosome with mRNA and tRNA

Recent collection of high-resolution crystal structures of the 70S ribosome with mRNA and tRNA substrates enhances our knowledge of protein synthesis principles. A novel network of interactions between the ribosome in the elongation state and mRNA downstream from the A codon suggests that mRNA is st...

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Bibliographic Details
Published in:Current opinion in structural biology 2010-06, Vol.20 (3), p.325-332
Main Authors: Demeshkina, Natalia, Jenner, Lasse, Yusupova, Gulnara, Yusupov, Marat
Format: Article
Language:English
Subjects:
Base Sequence
Codon - genetics
Codon - metabolism
Life Sciences
Ribosomes - chemistry
Ribosomes - metabolism
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Transfer - chemistry
RNA, Transfer - genetics
RNA, Transfer - metabolism
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Summary:Recent collection of high-resolution crystal structures of the 70S ribosome with mRNA and tRNA substrates enhances our knowledge of protein synthesis principles. A novel network of interactions between the ribosome in the elongation state and mRNA downstream from the A codon suggests that mRNA is stabilized and aligned at the entrance to the decoding center. The X-ray studies clarify how natural modifications of tRNA are involved in the stabilization of the codon–anticodon interactions, prevention of frame-shifting and also expansion of the decoding capacity of tRNAs. In addition, the crystal structures provide the view that tRNA in the A and P sites communicate through a protein rich environment and suggest how these tRNAs are controlled through the intersubunit bridge formed by protein L31.
ISSN:0959-440X
1879-033X
DOI:10.1016/j.sbi.2010.03.002