Autophagic event and metabolomic disorders unveil cellular toxicity of environmental microplastics on marine polychaete Hediste diversicolor

Although the hazards of microplastics (MPs) have been quite well explored, the aberrant metabolism and the involvement of the autophagy pathway as an adverse response to environmental MPs in benthic organisms are still unclear. The present work aims to assess the impact of different environmental MP...

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Published in:Environmental pollution (1987) 2022-06, Vol.302 (8), p.119106-119106, Article 119106
Main Authors: Missawi, Omayma, Venditti, Massimo, Cappello, Tiziana, Zitouni, Nesrine, Marco, Giuseppe DE, Boughattas, Iteb, Bousserrhine, Noureddine, Belbekhouche, Sabrina, Minucci, Sergio, Maisano, Maria, Banni, Mohamed
Format: Article
Language:English
Subjects:
Autophagy
Chemical Sciences
Cytoskeleton
Environmental microplastic
Metabolome
Polychaete
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Summary:Although the hazards of microplastics (MPs) have been quite well explored, the aberrant metabolism and the involvement of the autophagy pathway as an adverse response to environmental MPs in benthic organisms are still unclear. The present work aims to assess the impact of different environmental MPs collected from the south coast of the Mediterranean Sea, composed by polyethylene (PE), polyethylene vinyl acetate (PEVA), low-density polyethylene (LDPE), high-density polyethylene (HDPE), polypropylene (PP) and polyamide (PA) on the metabolome and proteome of the marine polychaete Hediste diversicolor. As a result, all the microplastic types were detected with Raman microspectroscopy in polychaetes tissues, causing cytoskeleton damage and induced autophagy pathway manifested by immunohistochemical labeling of specific targeted proteins, through Tubulin (Tub), Microtubule-associated protein light chain 3 (LC3), and p62 (also named Sequestosome 1). Metabolomics was conducted to further investigate the metabolic alterations induced by the environmental MPs-mixture in polychaetes. A total of 28 metabolites were differentially expressed between control and MPs-treated polychaetes, which showed elevated levels of amino acids, glucose, ATP/ADP, osmolytes, glutathione, choline and phosphocholine, and reduced concentration of aspartate. These novel findings extend our understanding given the toxicity of environmental microplastics and unravel their underlying mechanisms. [Display omitted] •H. diversicolor polychaetes were exposed to a cocktail of environmental MPs.•Environmental MPs were associated with autophagy and cytoskeleton alteration.•Environmental MPs trigger metabolome disorders.
ISSN:0269-7491
1873-6424
DOI:10.1016/j.envpol.2022.119106