Effect of DHA on the quality of In vitro produced bovine embryos

• Published in: Theriogenology 2022-07, Vol.187, p.102-111
• Main Authors: Janati Idrissi, Sarah, Slezec-Frick, Victoria, Le Bourhis, Daniel, Le Berre, Laurene, Joly, Thierry, Buff, Samuel, Desmarchais, Alice, Schibler, Laurent, Salvetti, Pascal, Elis, Sebastien
• Format: Article
• Language: English
• Subjects: Agricultural sciences; Animal biology; Cattle; Cryopreservation; Development Biology; Embryology and Organogenesis; Food and Nutrition; IVP; Life Sciences; n-3 PUFA; Reproductive Biology; Sexual reproduction; SOX2; Veterinary medicine and animal Health; Zootechny
• ISSN: 0093-691X; 1879-3231; 0093-691X
• DOI: 10.1016/j.theriogenology.2022.04.026

Docosahexaenoic acid (DHA) is an n-3 polyunsaturated fatty acid (PUFA) that improves fertility by increasing membrane fluidity. Moreover, embryos produced by donor females supplied with n-3 PUFA did not show any difference in terms of the lipid profile after 7 days of culture. The present study aimed to investigate the effects of DHA (20 and 100 μM) coupled with carnosine (5 mg/mL), an antioxidant, during oocyte maturation and embryo development on the developmental and cryosurvival rates and the number of pluripotent cells. Free fatty acid receptor-4 (FFAR4), which is able to bind DHA, was visualised by immunostaining. The addition of DHA in the in vitro development (IVD) medium decreased the percentage of pluripotent SOX2 positive cells compared with the control (8.4% vs. 10.9%) without affecting the number of cells (196.7 vs. 191.6 cells) or the developmental (20.9% vs. 23.9% blastocysts rate on D7) and cryosurvival rates (86.3% vs 86.2%). Such a decrease in pluripotent cells, relevant to the differentiation of the first lineage within the inner cell mass, represents an improvement in the embryo quality. On the contrary, embryos without any pluripotent SOX2-positive cells would not be able to achieve gestation. Future studies should follow up these results by carrying out embryo transfers to assess the beneficial effects of DHA supplementation. •Embryos that do no express SOX2 cannot lead to pregnancy.•A reduction in pluripotent SOX2 positive cells indicates indicates embryo differentiation.•DHA supplementation during IVD decreased SOX2 positive cell rate.•DHA tends to increase the percentage of embryos with SOX2 positive cells.