Phytochemical and biological activities of Bituminaria bituminosa L. (Fabaceae)

To investigate the phytochemical composition, the antioxidant and antibacterial activities of Bituminaria bituminosa L. (Fabaceae) (B. bituminosa). The aerial parts of B. bituminosa yielded two compounds. The structures of these compounds were determinated using UV, 1H-NMR and 13C-NMR experiments an...

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Bibliographic Details
Published in:Asian Pacific journal of tropical medicine 2014-09, Vol.7, p.S481-S484
Main Authors: Azzouzi, Salima, Zaabat, Nabila, Medjroubi, Kamel, Akkal, Salah, Benlabed, Kadour, Smati, Farida, Dijoux-Franca, Marie-Geneviève
Format: Article
Language:English
Subjects:
Antibacterial
Antioxidant
Bituminaria bituminosa L
Escherichia coli
Fabaceae
Flavone
Isoflavone
Klebsiella
Klebsiella pneumonia
Life Sciences
Pharmaceutical sciences
Staphylococcus aureus
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Summary:To investigate the phytochemical composition, the antioxidant and antibacterial activities of Bituminaria bituminosa L. (Fabaceae) (B. bituminosa). The aerial parts of B. bituminosa yielded two compounds. The structures of these compounds were determinated using UV, 1H-NMR and 13C-NMR experiments and comparison of their spectroscopic properties with literature data. The antibacterial activity of the extracts (CH2Cl2, ethyl acetate and n-BuOH) was determinated using disk diffusion method against standard and clinical strains. Antioxidant potential of n-BuOH extract was evaluated through two methods: DPPH and cupric ion reducing antioxidant capacity assay. The n-BuOH extract from B. bituminosa yielded the isolation of isoflavone and flavone. The extracts CH2Cl2, ethyl acetate and n-BuOH demonstrated significant antibacterial activities. CH2Cl2 extract showed the maximum antibacterial activity with high concentration of 2 mg/mL against Staphylococcus aureus ATCC 29213, Klebsiella pneumonia and Escherichia coli ATCC 25922 (20.45 mm, 16.41 mm and 15.74 mm inhibition zone, respectively). The value IC50 was 0.26 μg/mL for n-BuOH extract using DPPH method. Whereas the E% value was 0.10 L/mg every centimeter for cupric ion reducing antioxidant capacity assay. The phytochemical study of B. bituminosa revealed the presence of isoflavone (daidzin) and flavone (isoorientin) and identified for the first time in this specie. The antibacterial activity of the plant B. bituminosa is certainly related to its chemical content. The n-BuOH extract showed a significant antioxidant activity.
ISSN:1995-7645
2352-4146
DOI:10.1016/S1995-7645(14)60278-9