Understanding the role of surface interactions in the antibacterial activity of layered double hydroxide nanoparticles by atomic force microscopy

• Published in: Nanoscale 2022-07, Vol.14 (29), p.1335-1348
• Main Authors: Awassa, Jazia, Soulé, Samantha, Cornu, Damien, Ruby, Christian, El-Kirat-Chatel, Sofiane
• Format: Article
• Language: English
• Subjects: Antibiotics; Atomic force microscopy; Bacteriology; Chemical Sciences; Dissolution; Hydroxides; Life Sciences; Material chemistry; Microbiology and Parasitology; Microscopy; Mycology; Nanoparticles; Spectrum analysis; Zinc
• ISSN: 2040-3364; 2040-3372
• DOI: 10.1039/d2nr02395d

Understanding the mechanisms of the interactions between zinc-based layered double hydroxides (LDHs) and bacterial surfaces is of great importance to improve the efficiency of these antibiotic-free antibacterial agents. In fact, the role of surface interactions in the antibacterial activity of zinc-based LDH nanoparticles compared to that of dissolution and generation of reactive oxygen species (ROS) is still not well documented. In this study, we show that ZnAl LDH nanoparticles exhibit a strong antibacterial effect against Staphylococcus aureus by inducing serious cell wall damages as revealed by the antibacterial activity tests and atomic force microscopy (AFM) imaging, respectively. The comparison of the antibacterial properties of ZnAl LDH nanoparticles and micron-sized ZnAl LDHs also demonstrated that the antibacterial activity of Zn-based LDHs goes beyond the simple dissolution into Zn 2+ antibacterial ions. Furthermore, we developed an original approach to functionalize AFM tips with LDH films in order to probe their interactions with living S. aureus cells by means of AFM-based force spectroscopy (FS). The force spectroscopy analysis revealed that antibacterial ZnAl LDH nanoparticles show specific recognition of S. aureus cells with high adhesion frequency and remarkable force magnitudes. This finding provides a first insight into the antibacterial mechanism of Zn-based LDHs through direct surface interactions by which they are able to recognize and adhere to bacterial surfaces, thus damaging them and leading to subsequent growth inhibition. Understanding the mechanisms of the interactions between zinc-based layered double hydroxides (LDHs) and bacterial surfaces is of great importance to improve the efficiency of these antibiotic-free antibacterial agents.