Bacteriophages vehiculate a high amount of antibiotic resistance determinants of bacterial origin in the Orne River ecosystem

Summary Aquatic environments are important dissemination routes of antibiotic resistance genes (ARGs) from and to pathogenic bacteria. Nevertheless, in these complex matrices, identifying and characterizing the driving microbial actors and ARG dissemination mechanisms they are involved in remain dif...

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Bibliographic Details
Published in:Environmental microbiology 2022-09, Vol.24 (9), p.4317-4328
Main Authors: Sagrillo, Charlène, Changey, Frédérique, Bellanger, Xavier
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Aquatic ecosystems
Aquatic environment
Bacteria
Bacteriology
Bacteriophages
Biodiversity
Biodiversity and Ecology
Chromosomes
Deoxyribonucleic acid
Disease resistance
Distribution
DNA
Drug resistance
Environmental Sciences
Gene transfer
Genes
Horizontal transfer
Life Sciences
Microbiology and Parasitology
Microorganisms
Pathogenic bacteria
Phages
Populations and Evolution
Rivers
rRNA 16S
Seasonal variations
Seasonality
Sediment
Sediments
Transduction
Virology
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Summary:Summary Aquatic environments are important dissemination routes of antibiotic resistance genes (ARGs) from and to pathogenic bacteria. Nevertheless, in these complex matrices, identifying and characterizing the driving microbial actors and ARG dissemination mechanisms they are involved in remain difficult. We here explored the distribution/compartmentalization of a panel of ARGs and mobile genetic elements (MGEs) in bacteria and bacteriophages collected in the water, suspended material and surface sediments from the Orne River ecosystem (France). By using a new bacteriophage DNA extraction method, we showed that, when packaging bacterial DNA, bacteriophages rather encapsidate both ARGs and MGEs than 16S rRNA genes, i.e. chromosomal fragments. We also show that the bacteria and bacteriophage capsid contents in ARGs/MGEs were similarly influenced by seasonality but that the distribution of ARGs/MGEs between the river physical compartments (water vs. suspended mater vs. sediment) is more impacted when these markers were carried by bacteria. These demonstrations will likely modify our understanding of the formation and fate of transducing viral particles in the environment. Consequently, they will also likely modify our estimations of the relative frequencies of the different horizontal gene transfer mechanisms in disseminating antibiotic resistance by reinforcing the roles played by environmental bacteriophages and transduction.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.16083