Determinants of a bronchoalveolar lavage of good quality for mineralogical analyses in adults: Experience from the Asbestos Fibers and Particles Laboratory of Paris City

• Published in: Respiratory medicine and research 2022-05, Vol.81, p.100885-100885, Article 100885
• Main Authors: Fadel, Marc, Tran Van Nhieu, Jeanne, Grall, Pierre, Beugnon, Karine, Boudet, Perrine, Schlemmer, Frédéric, Mangiapan, Gilles, Maitre, Bernard, Boczkowski, Jorge, Martinon, Laurent, Pairon, Jean-Claude
• Format: Article
• Language: English
• Subjects: Asbestos; Bronchoalveolar lavage; Cytology; Life Sciences; Occupational diseases; Silicosis
• ISSN: 2590-0412; 2590-0412
• DOI: 10.1016/j.resmer.2022.100885

Mineralogical analyses of bronchoalveolar lavage (BAL) may help in assessing past exposure to mineral particles. However, their interpretation relies on their quality, meaning their representativeness of the alveolar compartment. The aim of this study was to find predictive factors of BAL samples quality allowing a reliable mineralogical analysis. All BAL samples analyzed between 2018 and 2020 in the Asbestos Fibers and Particles Laboratory from Paris City were included. They were read by an experienced cyto-pathologist and validated according to their representativeness of the alveolar region compartment. Univariate and stratified analyses were conducted to identify factors associated with the samples’ cytological quality. On the 780 samples included, 64.4% were deemed of good cytological quality and 17.9% were not interpretable. Injected volume and BAL yield (recovery volume on injected volume ratio) were associated with cytological quality. Injecting at least 100mL with a ≥60% yield or injecting at least 150mL with a ≥30% yield allowed having a good proportion of BAL with sufficient cytological quality. Injected volume greater than 100mL with sufficient BAL yield are essential factors to ensure a reliable mineralogical analysis of BAL samples.