Lipid peroxidation in cotton: Xanthomonas interactions and the role of lipoxygenases during the hypersensitive reaction

• Published in: The Plant journal : for cell and molecular biology 2002-10, Vol.32 (1), p.1-12
• Main Authors: Jalloul, A., Montillet, J. L., Assigbetsé, K., Agnel, J.P., Delannoy, E., Triantaphylidès, C., Daniel, J.F., Marmey, P., Geiger, J.P., Nicole, M.
• Format: Article
• Language: English
• Subjects: Apoptosis - physiology; Bacterial plant pathogens; Biological and medical sciences; cotton; Cotyledon - genetics; Cotyledon - metabolism; Cotyledon - microbiology; Fatty Acids, Unsaturated - biosynthesis; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control; Gossypium - genetics; Gossypium - metabolism; Gossypium - microbiology; hypersensitive response; Immunity, Innate - genetics; Life Sciences; lipid peroxidation; Lipid Peroxidation - physiology; lipoxygenase; Lipoxygenase - genetics; Lipoxygenase - metabolism; Molecular Sequence Data; Oxidative Stress - physiology; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Reactive Oxygen Species - metabolism; Signal Transduction; Water - metabolism; Xanthomonas; Xanthomonas - growth & development
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.2002.01393.x

Summary Lipid peroxidation, often associated with hypersensitive cell death, may be initiated either by active oxygen species (AOS) or lipoxygenases (LOX). Here we report a detailed analysis of this oxidative process in both incompatible and compatible interactions between the cotton cultivar Reba B50 and Xanthomonas campestris pv. malvacearum (Xcm). The hypersensitive reaction (HR) was characterized by a massive production of polyunsaturated fatty acid (PUFA) hydroperoxides together with typical tissue dehydration. Among these, isomers peroxidized on carbon 9, largely predominant, were chiral, showing an excess in the S enantiomer. The HR process was accompanied by an increase in 9S‐LOX activity and preceded by transcription of a LOX gene (GhKLox1). These results showed that: (i) AOS produced during the oxidative burst were not involved in PUFA peroxidation during HR; and (ii) as previously described in elicited leaves of tobacco, the massive enzymatic lipid peroxidation was closely associated with hypersensitive cell death. During disease development in this cotton cultivar, the 9‐lipoxygenation of PUFAs was late, weak, preceded by a faint accumulation of GhKLox1 transcripts, and associated with chlorosis but not with necrosis. Consequently, the main difference between incompatible and compatible interactions was in the precocity and intensity of the oxidative process, rather than in its nature. These data provide the evidence for a correlation between lipid peroxidation and hypersensitive cell death induced by pathogens.