Structure-function-dynamics relationships in the peculiar Planktothrix PCC7805 OCP1: Impact of his-tagging and carotenoid type

The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that this OCP variant is characterized by higher photoac...

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Published in:Biochimica et biophysica acta. Bioenergetics 2022-10, Vol.1863 (7), p.148584-148584, Article 148584
Main Authors: Wilson, Adjélé, Andreeva, Elena A., Niziński, Stanisław, Talbot, Léa, Hartmann, Elisabeth, Schlichting, Ilme, Burdzinski, Gotard, Sliwa, Michel, Kirilovsky, Diana, Colletier, Jacques-Philippe
Format: Article
Language:English
Subjects:
Bacterial Proteins
Biochemistry, Molecular Biology
Carotenoids
Cyanobacteria
Flash photolysis
Fluorescence
Life Sciences
Photosynthetic pigments
Planktothrix
Structural Biology
Structure function relationships
X-ray diffraction
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Summary:The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that this OCP variant is characterized by higher photoactivation and recovery rates, and a stronger energy-quenching activity, compared to other OCP studied thus far. We characterize the effect of the functionalizing carotenoid and of his-tagging on these reactions, and identify the time scales on which these modifications affect photoactivation. The presence of a his-tag at the C-terminus has a large influence on photoactivation, thermal recovery and PBS-fluorescence quenching, and likewise for the nature of the carotenoid that additionally affects the yield and characteristics of excited states and the ns-s dynamics of photoactivated OCP. By solving the structures of Plankto-OCP in the ECN- and CAN-functionalized states, each in two closely-related crystal forms, we further unveil the molecular breathing motions that animate Plankto-OCP at the monomer and dimer levels. We finally discuss the structural changes that could explain the peculiar properties of Plankto-OCP. •Complete functional characterization of Synechocystis and Planktothrix OCP•Hitherto unknown structures of ECN- and CAN-functionalized Planktothrix OCP•Insights into fs-s timescale photodynamics of ECN- and CAN-functionalized Synechocystis and Planktothrix OCP
ISSN:0005-2728
1879-2650
DOI:10.1016/j.bbabio.2022.148584