EPR-MRS site: EPR spectroscopy for the study of biomolecules

Electron Paramagnetic Resonance (EPR) spectroscopy is the method of choice for the study of paramagnetic species such as radicals and transition metal ions. In Life sciences, it is of great use for the study of the active sites of metalloproteins [1], the function of complex redox systems [2], prote...

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Bibliographic Details
Published in:Biochimica et biophysica acta. Bioenergetics 2022-09, Vol.1863, p.148685, Article 148685
Main Authors: Dorlet, Pierre, Gerbaud, Guillaume, Etienne, Emilien, Grimaldi, Stéphane, Guigliarelli, Bruno, Belle, Valérie
Format: Article
Language:English
Subjects:
Biochemistry
Biochemistry, Molecular Biology
Biophysics
Catalysis
Chemical Sciences
Coordination chemistry
Electromagnetism
Engineering Sciences
Inorganic chemistry
Life Sciences
Structural Biology
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Summary:Electron Paramagnetic Resonance (EPR) spectroscopy is the method of choice for the study of paramagnetic species such as radicals and transition metal ions. In Life sciences, it is of great use for the study of the active sites of metalloproteins [1], the function of complex redox systems [2], protein dynamics [3, 4] or protein-protein interactions [5]. The EPR facility hosted in the laboratory of Bioenergetics and Engineering of Proteins in Marseille (https://bip.cnrs.fr/epr-facility/) is one of the four French EPR centers included in the National Research Infrastructure INFRANALYTICS (FR2054) and comprises several high-end spectrometers for both continuous-wave or pulse EPR experiments at microwave frequencies ranging from X-band (9 GHz) to Q-band (35 GHz) and W-band (95 GHz). The application of EPR to biological systems will be illustrated by examples of ongoing studies and research projects we have welcomed in the frame of the TNA of the European MOSBRI Infrastructure. Access to the EPR-MRS facilities can be provided free of charge through the French and European research infrastructures INFRANALYTICS (www.infranalytics.cnrs.fr) and MOSBRI (www. mosbri.eu), respectively.
ISSN:0005-2728
1879-2650
DOI:10.1016/j.bbabio.2022.148685