Impact of high-fat diet on the intestinal microbiota and small intestinal physiology before and after the onset of obesity

• Published in: Biochimie 2017-10, Vol.141, p.97-106
• Main Authors: Araújo, João Ricardo, Tomas, Julie, Brenner, Christiane, Sansonetti, Philippe J.
• Format: Article
• Language: English
• Subjects: Animals; Dietary Fats - adverse effects; Dietary Fats - immunology; Fat; Food and Nutrition; Gastrointestinal Microbiome - immunology; Humans; Inflammation; Intestine; Intestine, Small - immunology; Intestine, Small - microbiology; Life Sciences; Microbiology and Parasitology; Microbiota; Mucus; Obesity; Obesity - immunology; Obesity - microbiology; Toll-Like Receptor 4 - immunology
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/j.biochi.2017.05.019

The modulation of the intestinal microbiota by high-fat diet (HFD) has a major impact on both immunological and metabolic functions of the host. Taking this into consideration, the aim of this contribution is to review the impact of HFD on microbiota profile and small intestinal physiology before and after the onset of obesity and its metabolic complications. Evidence from animal studies suggest that before the onset of obesity and its metabolic complications, HFD induces intestinal dysbiosis - encompassing changes in composition balance and massive redistribution with bacteria occupying intervillous spaces and crypts - associated with early physiopathological changes, predominantly in the ileum, such as low-grade inflammation, decreased antimicrobial peptides expression, impaired mucus production, secretion and layer's thickness, and decreased expression of tight junction proteins. With time, major inflammatory signals (e.g. toll-like receptor-4 dependent) become activated, thereby stimulating proinflammatory cytokines secretion in the small intestine. This inflammatory state might subsequently exacerbate disruption of the mucus layer barrier and increase epithelial permeability of the small intestine, thereby creating an environment that facilitates the passage of bacterial components (e.g. lipopolysaccharide, peptidoglycan and flagellin) and metabolites from the intestinal lumen (e.g. secondary bile acids) to the circulation and peripheral tissues (i.e. leaky gut), eventually promoting the development of systemic inflammation, obesity, adiposity, insulin resistance and glucose intolerance preceding hyperglycemia. Although the mechanisms are still not completely understood, prebiotics, probiotics, polyphenols, peroxisome proliferator-activated receptor-γ agonists (such as rosiglitazone) and exercise have been shown to reverse HFD-induced intestinal phenotype and to attenuate the severity of obesity and its associated metabolic complications. •High-fat diet (HFD) impacts immunological and metabolic functions of the host.•Gut dysbiosis, inflammation and leakage are induced by HFD consumption.•These changes seem to be associated the development of obesity and its complications.•Some prebiotics, probiotics and antidiabetics attenuate this HFD-induced phenotype.