Mapping of QTLs for citrus quality traits throughout the fruit maturation process on clementine (Citrus reticulata × C. sinensis) and mandarin (C. reticulata Blanco) genetic maps

• Published in: Tree genetics & genomes 2022-12, Vol.18 (6), Article 40
• Main Authors: Khefifi, Hajer, Dumont, Doriane, Costantino, Gilles, Doligez, Agnès, Brito, Anna Carla, Bérard, Aurélie, Morillon, Raphael, Ollitrault, Patrick, Luro, François
• Format: Article
• Language: English
• Subjects: Acidity; Agricultural sciences; Biomedical and Life Sciences; Biotechnology; Citrus fruits; Citrus reticulata; Citrus sinensis; Dry matter; Forestry; Fructose; Fruits; Gene mapping; Genetic control; Genetic crosses; Glucose; Heredity; Hybrids; Life Sciences; Localization; Malic acid; Mandarins; Markers; Maturation; Original Article; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Progeny; Quantitative trait loci; Ripening; Single-nucleotide polymorphism; Sucrose; Tree Biology
• ISSN: 1614-2942; 1614-2950
• DOI: 10.1007/s11295-022-01571-w

Citrus fruit quality is defined as the combination of physical and chemical traits; some of which may change during the ripening phase, e.g., acidity and sugar content. A clear understanding of their genetic control would be very helpful for marker-assisted breeding programs especially with regard to the juvenile phase and some reproductive features that hamper the selection of improved hybrids. A genetic study was thus performed on the heredity of quality traits and QTL detection based on segregation in a progeny generated from a cross between clementine cv “Commun” ( Citrus  ×  reticulata cv clementine ) and mandarin cv “Willow leaf” ( C. reticulata Blanco). Parental and consensus genetic linkage maps were constructed using 645 SNP and SSR markers. These maps were represented by 10 linkage groups in clementine and 12 linkage groups in mandarin, representing 75% and 58% respectively of the previously published clementine reference map. A total of 16 traits, including fruit mass, equatorial diameter, juice percentage, total soluble solids, acidity, pH, glucose, fructose, sucrose, and citric and malic acid concentrations were evaluated at three maturation dates. High variations indicating transgressive segregation were found for all traits, with normal or close to normal distributions. QTL analysis performed using the multiple QTL model allowed the detection of 34 QTLs on the three maps. QTLs were distributed in different linkage groups and generally detected at only one date of the ripening phase. The percentage of total variation explained ranged from 12 to 37% per QTL. Major QTLs ( R 2  ≥ 30%) were detected for equatorial diameter, glucose, and fructose (expressed in percentage dry matter) on linkage groups 8 and 9. Co-localization of QTLs controlling correlated and uncorrelated traits were mainly found on linkage groups 2, 4, 8, and 9, particularly between fruit mass and acidity.