Dual Role of Vitamin C on the Neuroinflammation Mediated Neurodegeneration and Memory Impairments in Colchicine Induced Rat Model of Alzheimer Disease

• Published in: Journal of molecular neuroscience 2016-12, Vol.60 (4), p.421-435
• Main Authors: Sil, Susmita, Ghosh, Tusharkanti, Gupta, Pritha, Ghosh, Rupsa, Kabir, Syed N, Roy, Avishek
• Format: Article
• Language: English
• Subjects: Alzheimer Disease - drug therapy; Alzheimer Disease - etiology; Alzheimer's disease; Animals; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Ascorbic Acid - administration & dosage; Ascorbic Acid - pharmacology; Ascorbic Acid - therapeutic use; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cognitive science; Colchicine - toxicity; Cytokines; Experiments; Hippocampus - drug effects; Hippocampus - metabolism; Inflammation; Inflammation - drug therapy; Lipid peroxidation; Male; Memory; Memory - drug effects; Mice; Neurochemistry; Neurodegeneration; Neurology; Neuroscience; Neurosciences; Oxidative Stress; Physiology; Proteomics; Rats; Reactive Oxygen Species - metabolism; Recovery (Medical); Surgery; Vitamin C; Vitamins - administration & dosage; Vitamins - pharmacology; Vitamins - therapeutic use
• ISSN: 0895-8696; 1559-1166
• DOI: 10.1007/s12031-016-0817-5

The neurodegeneration in colchicine induced AD rats (cAD) is mediated by cox-2 linked neuroinflammation. The importance of ROS in the inflammatory process in cAD has not been identified, which may be deciphered by blocking oxidative stress in this model by a well-known anti-oxidant vitamin C. Therefore, the present study was designed to investigate the role of vitamin C on colchicine induced oxidative stress linked neuroinflammation mediated neurodegeneration and memory impairments along with peripheral immune responses in cAD. The impairments of working and reference memory were associated with neuroinflammation and neurodegeneration in the hippocampus of cAD. Administration of vitamin C (200 and 400 mg/kg BW) in cAD resulted in recovery of memory impairments, with prevention of neurodegeneration and neuroinflammation in the hippocampus. The neuroinflammation in the hippocampus also influenced the peripheral immune responses and inflammation in the serum of cAD and all of these parameters were also recovered at 200 and 400 mg dose of vitamin C. However, cAD treated with 600 mg dose did not recover but resulted in increase of memory impairments, neurodegeneration and neuroinflammation in hippocampus along with alteration of peripheral immune responses in comparison to cAD of the present study. Therefore, the present study showed that ROS played an important role in the colchicine induced neuroinflammation linked neurodegeneration and memory impairments along with alteration of peripheral immune responses. It also appears from the results that vitamin C at lower doses showed anti-oxidant effect and at higher dose resulted in pro-oxidant effects in cAD.