In mice and humans, brain microvascular contractility matures postnatally

Although great efforts to characterize the embryonic phase of brain microvascular system development have been made, its postnatal maturation has barely been described. Here, we compared the molecular and functional properties of brain vascular cells on postnatal day (P)5 vs. P15, via a transcriptom...

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Published in:Brain Structure and Function 2023-03, Vol.228 (2), p.475-492
Main Authors: Slaoui, Leila, Gilbert, Alice, Rancillac, Armelle, Delaunay-Piednoir, Barbara, Chagnot, Audrey, Gerard, Quentin, Letort, Gaëlle, Mailly, Philippe, Robil, Noémie, Gelot, Antoinette, Lefebvre, Mathilde, Favier, Maryline, Dias, Karine, Jourdren, Laurent, Federici, Laetitia, Auvity, Sylvain, Cisternino, Salvatore, Vivien, Denis, Cohen-Salmon, Martine, Boulay, Anne-Cécile
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Biomedical and Life Sciences
Biomedicine
Blood flow
Blood vessels
Brain - blood supply
Brain research
Cell Biology
Cerebral blood flow
Critical period
Embryogenesis
Endothelial Cells
Fibroblasts
Gene expression
Humans
Life Sciences
Maturation
Mice
Microvasculature
Muscle Contraction
Muscle, Smooth, Vascular - physiology
Neurology
Neurosciences
Original Article
Smooth muscle
Transcriptomics
Veins & arteries
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Summary:Although great efforts to characterize the embryonic phase of brain microvascular system development have been made, its postnatal maturation has barely been described. Here, we compared the molecular and functional properties of brain vascular cells on postnatal day (P)5 vs. P15, via a transcriptomic analysis of purified mouse cortical microvessels (MVs) and the identification of vascular-cell-type-specific or -preferentially expressed transcripts. We found that endothelial cells (EC), vascular smooth muscle cells (VSMC) and fibroblasts (FB) follow specific molecular maturation programs over this time period. Focusing on VSMCs, we showed that the arteriolar VSMC network expands and becomes contractile resulting in a greater cerebral blood flow (CBF), with heterogenous developmental trajectories within cortical regions. Samples of the human brain cortex showed the same postnatal maturation process. Thus, the postnatal phase is a critical period during which arteriolar VSMC contractility required for vessel tone and brain perfusion is acquired and mature.
ISSN:1863-2661
1863-2653
1863-2661
0340-2061
DOI:10.1007/s00429-022-02592-w