Genes encoding subunit 6 of NADH dehydrogenase and subunit 6 of ATP synthase are co-transcribed in maize mitochondria

The mitochondrial nad6 gene of maize was identified and mapped 1 kb downstream from the atp6 gene. It encodes a 220 amino-acid polypeptide. Using Northern hybridization experiments and RT-PCR analysis, we showed that both nad6 and atp6 are co-transcribed in maize mitochondria. RNA editing of the mit...

Full description

Saved in:
Bibliographic Details
Published in:Current genetics 1997-01, Vol.31 (1), p.63-69
Main Authors: Haouazine-Takvorian, N, Takvorian, A, Jubier, M.F, Lejeune, B. (Universite Paris 11, URA CNRS, Orsay (France). Inst. de Biotechnologie des Plantes)
Format: Article
Language:English
Subjects:
ADENOSINE TRIPHOSPHATE
ADENOSINTRIFOSFATO
Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Southern
Brassica - genetics
Chromosome Mapping
Cloning, Molecular
DNA, Complementary - genetics
GENE
GENES
GENETICA MITOCONDRIAL
GENETIQUE MITOCHONDRIALE
Life Sciences
LIGASAS
LIGASE
LIGASES
Mitochondria - enzymology
Mitochondria - genetics
MITOCHONDRIAL GENETICS
Molecular Sequence Data
NADH Dehydrogenase - genetics
Open Reading Frames
OXIDOREDUCTASES
OXIDORREDUCTASAS
OXYDOREDUCTASE
Plant Proteins - genetics
Polymerase Chain Reaction
Proton-Translocating ATPases
RNA Processing, Post-Transcriptional
Sequence Alignment
Sequence Analysis, DNA
TRANSCRIPCION
TRANSCRIPTION
Transcription, Genetic
Triticum - genetics
ZEA MAYS
Zea mays - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mitochondrial nad6 gene of maize was identified and mapped 1 kb downstream from the atp6 gene. It encodes a 220 amino-acid polypeptide. Using Northern hybridization experiments and RT-PCR analysis, we showed that both nad6 and atp6 are co-transcribed in maize mitochondria. RNA editing of the mitochondrial nad6 transcript was studied by cDNA sequencing. Twelve edited sites were identified at the same positions as those already identified in the wheat mitochondria nad6 transcript. Alignments of nucleotide and amino-acid sequences of the mitochondrial nad6 genes of maize, wheat, and Brassica campestris, show that the wheat gene encodes a shorter polypeptide (229 amino acids) than was previously thought.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050177