Diversity of photosynthetic picoeukaryotes in eutrophic shallow lakes as assessed by combining flow cytometry cell-sorting and high throughput sequencing

Photosynthetic picoeukaryotes (PPE) are key components of primary production in marine and freshwater ecosystems. In contrast with those of marine environments, freshwater PPE groups have received little attention. In this work, we used flow cytometry cell sorting, microscopy and metabarcoding to in...

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Bibliographic Details
Published in:FEMS microbiology ecology 2019-05, Vol.95 (5), p.1
Main Authors: Metz, Sebastián, Lopes Dos Santos, Adriana, Berman, Manuel Castro, Bigeard, Estelle, Licursi, Magdalena, Not, Fabrice, Lara, Enrique, Unrein, Fernando
Format: Article
Language:English
Subjects:
Aquatic ecosystems
Argentina
Biodiversity
Chlorophyta - classification
Chlorophyta - cytology
Chlorophyta - genetics
Chlorophyta - metabolism
Diatoms - classification
Diatoms - genetics
Diatoms - isolation & purification
Diatoms - metabolism
DNA sequencing
Ecology
Ecosystem
Ecosystems
Environmental Sciences
Eukaryota - classification
Eukaryota - genetics
Eukaryota - isolation & purification
Eukaryota - metabolism
Eukaryotes
Eutrophic lakes
Eutrophication
Flow Cytometry
Freshwater ecosystems
Genetic aspects
High-Throughput Nucleotide Sequencing
Lakes
Lakes - analysis
Lakes - parasitology
Marine ecosystems
Marine environment
Methods
Microbiology
Microscopy
Next-generation sequencing
Novels
Observations
Parasites
Photosynthesis
Phylogeny
Plankton
Populations
Primary production
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Summary:Photosynthetic picoeukaryotes (PPE) are key components of primary production in marine and freshwater ecosystems. In contrast with those of marine environments, freshwater PPE groups have received little attention. In this work, we used flow cytometry cell sorting, microscopy and metabarcoding to investigate the composition of small photosynthetic eukaryote communities from six eutrophic shallow lakes in South America, Argentina. We compared the total molecular diversity obtained from PPE sorted populations as well as from filtered total plankton samples (FTP). Most reads obtained from sorted populations belonged to the classes: Trebouxiophyceae, Chlorophyceae and Bacillariophyceae. We retrieved sequences from non-photosynthetic groups, such as Chytridiomycetes and Ichthyosporea which contain a number of described parasites, indicating that these organisms were probably in association with the autotrophic cells sorted. Dominant groups among sorted PPEs were poorly represented in FTP and their richness was on average lower than in the sorted samples. A significant number of operational taxonomic units (OTUs) were exclusively found in sorting samples, emphasizing that sequences from FTP underestimate the diversity of PPE. Moreover, 22% of the OTUs found among the dominant groups had a low similarity (
ISSN:1574-6941
0168-6496
1574-6941
DOI:10.1093/femsec/fiz038