Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization

Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone enc...

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Bibliographic Details
Published in:Gene 1995-12, Vol.167 (1), p.25-31
Main Authors: Schwaab, Véronique, Baud, Eric, Ghyselinck, Norbert, Mattei, Marie-Geneviève, Dufaure, Jean-Pierre, Drevet, Joël R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
antioxidant enzyme
Base Sequence
Biochemistry, Molecular Biology
Chromosome Mapping
Cloning, Molecular
Consensus Sequence
Epididymis - enzymology
Gene Expression
Genes
Glutathione Peroxidase - blood
Glutathione Peroxidase - genetics
GPX5
Life Sciences
Male
Mice
Molecular Sequence Data
Murine epididymis
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
Restriction Mapping
RNA, Messenger - genetics
selenoprotein
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Summary:Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone encoding mouse plasma GPX (GPX3). GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00551-X