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Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization

Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone enc...

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Published in:	Gene 1995-12, Vol.167 (1), p.25-31
Main Authors:	Schwaab, Véronique, Baud, Eric, Ghyselinck, Norbert, Mattei, Marie-Geneviève, Dufaure, Jean-Pierre, Drevet, Joël R.
Format:	Article
Language:	English
Subjects:	Amino Acid Sequence Animals antioxidant enzyme Base Sequence Biochemistry, Molecular Biology Chromosome Mapping Cloning, Molecular Consensus Sequence Epididymis - enzymology Gene Expression Genes Glutathione Peroxidase - blood Glutathione Peroxidase - genetics GPX5 Life Sciences Male Mice Molecular Sequence Data Murine epididymis Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Restriction Mapping RNA, Messenger - genetics selenoprotein
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cited_by	cdi_FETCH-LOGICAL-c391t-73a92bdf382a08c2670d648aa37c26169153f08d9c0f3d820575e5c8b9aac0a33
cites	cdi_FETCH-LOGICAL-c391t-73a92bdf382a08c2670d648aa37c26169153f08d9c0f3d820575e5c8b9aac0a33
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container_title	Gene
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creator	Schwaab, Véronique Baud, Eric Ghyselinck, Norbert Mattei, Marie-Geneviève Dufaure, Jean-Pierre Drevet, Joël R.
description	Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone encoding mouse plasma GPX (GPX3). GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.
doi_str_mv	10.1016/0378-1119(95)00551-X
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GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(95)00551-X</identifier><identifier>PMID: 8566787</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; antioxidant enzyme ; Base Sequence ; Biochemistry, Molecular Biology ; Chromosome Mapping ; Cloning, Molecular ; Consensus Sequence ; Epididymis - enzymology ; Gene Expression ; Genes ; Glutathione Peroxidase - blood ; Glutathione Peroxidase - genetics ; GPX5 ; Life Sciences ; Male ; Mice ; Molecular Sequence Data ; Murine epididymis ; Promoter Regions, Genetic ; Regulatory Sequences, Nucleic Acid ; Restriction Mapping ; RNA, Messenger - genetics ; selenoprotein</subject><ispartof>Gene, 1995-12, Vol.167 (1), p.25-31</ispartof><rights>1995</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-73a92bdf382a08c2670d648aa37c26169153f08d9c0f3d820575e5c8b9aac0a33</citedby><cites>FETCH-LOGICAL-c391t-73a92bdf382a08c2670d648aa37c26169153f08d9c0f3d820575e5c8b9aac0a33</cites><orcidid>0000-0003-4042-6818</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8566787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03993687$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Schwaab, Véronique</creatorcontrib><creatorcontrib>Baud, Eric</creatorcontrib><creatorcontrib>Ghyselinck, Norbert</creatorcontrib><creatorcontrib>Mattei, Marie-Geneviève</creatorcontrib><creatorcontrib>Dufaure, Jean-Pierre</creatorcontrib><creatorcontrib>Drevet, Joël R.</creatorcontrib><title>Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization</title><title>Gene</title><addtitle>Gene</addtitle><description>Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone encoding mouse plasma GPX (GPX3). GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>antioxidant enzyme</subject><subject>Base Sequence</subject><subject>Biochemistry, Molecular Biology</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>Consensus Sequence</subject><subject>Epididymis - enzymology</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Glutathione Peroxidase - blood</subject><subject>Glutathione Peroxidase - genetics</subject><subject>GPX5</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Murine epididymis</subject><subject>Promoter Regions, Genetic</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Restriction Mapping</subject><subject>RNA, Messenger - genetics</subject><subject>selenoprotein</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp9kUGLFDEQhYMo67j6DxRyEhdsTTqbTrIHYRnUFQa8KOwt1CTVM5F0Z0y6F_XXm3aGOXoKyfteFXmPkJecveOMd--ZULrhnJs3Rl4xJiVv7h-RFdfKNIwJ_ZiszshT8qyUH4wtWHtBLrTsOqXViszrmMYw7mjq6bRHOqS5IN3hiBRHl_wiHSKUAeguzhNM-5CqdsCcfgUPBW9oyjsYwx-YqvKWFvw5VydSGD11-5yGVNIAkcbkIJ6w5-RJD7Hgi9N5Sb5_-vhtfddsvn7-sr7dNE4YPjVKgGm3vhe6BaZd2ynmu2sNIFS98M5wKXqmvXGsF163TCqJ0umtAXAMhLgkV8e5e4j2kMMA-bdNEOzd7cYub0wYIzqtHnhlXx_ZQ071C2WyQygOY4QRayhWKS24ZLqC10fQ5VRKxv48mTO7NGOX2O0SuzXS_mvG3lfbq9P8eTugP5tOVVT9w1HHGshDwGyLC0uSPmR0k_Up_H_BXwX8npQ</recordid><startdate>19951229</startdate><enddate>19951229</enddate><creator>Schwaab, Véronique</creator><creator>Baud, Eric</creator><creator>Ghyselinck, Norbert</creator><creator>Mattei, Marie-Geneviève</creator><creator>Dufaure, Jean-Pierre</creator><creator>Drevet, Joël R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-4042-6818</orcidid></search><sort><creationdate>19951229</creationdate><title>Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization</title><author>Schwaab, Véronique ; 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GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8566787</pmid><doi>10.1016/0378-1119(95)00551-X</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-4042-6818</orcidid></addata></record>
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source	ScienceDirect Freedom Collection
subjects	Amino Acid Sequence Animals antioxidant enzyme Base Sequence Biochemistry, Molecular Biology Chromosome Mapping Cloning, Molecular Consensus Sequence Epididymis - enzymology Gene Expression Genes Glutathione Peroxidase - blood Glutathione Peroxidase - genetics GPX5 Life Sciences Male Mice Molecular Sequence Data Murine epididymis Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Restriction Mapping RNA, Messenger - genetics selenoprotein
title	Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization
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