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Amplification of blood smear DNA to confirm disseminated histoplasmosis
Background The prevalence of the Histoplasma capsulatum var. capsulatum ( Hcc ) histoplasmosis may be underestimated West Africa, both because the diagnosis is not mentioned in the early stages of the disease and due to limited biological resources available. Case Report We report a case of dissemin...
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| Published in: | Infection 2017-10, Vol.45 (5), p.687-690 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c406t-9247083ee8567cc5766b53005f7ec594468a2676545ee51071a6c41ee92bac603 |
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| cites | cdi_FETCH-LOGICAL-c406t-9247083ee8567cc5766b53005f7ec594468a2676545ee51071a6c41ee92bac603 |
| container_end_page | 690 |
| container_issue | 5 |
| container_start_page | 687 |
| container_title | Infection |
| container_volume | 45 |
| creator | Dieng, T. Massaly, A. Sow, D. Vellaissamy, S. Sylla, K. Tine, R. C. Dieng, Y. Hennequin, C. |
| description | Background
The prevalence of the
Histoplasma capsulatum
var.
capsulatum
(
Hcc
) histoplasmosis may be underestimated West Africa, both because the diagnosis is not mentioned in the early stages of the disease and due to limited biological resources available.
Case Report
We report a case of disseminated histoplasmosis due to
Hcc
in a Senegalese HIV patient. The diagnosis was suspected following the demonstration of small encapsulated yeasts within neutrophils on a thin blood smear. It was further confirmed using a specific real-time PCR applied on a DNA specimen extracted from the thin blood smear.
Conclusion
To the best of our knowledge, this is the first case of
Hcc
infection diagnosed in Senegal. Blood smear may be a valuable screening tool in the case of bloodstream dissemination and can be used for further molecular approaches to confirm the diagnosis. |
| doi_str_mv | 10.1007/s15010-017-0989-0 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_04002554v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1870645595</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-9247083ee8567cc5766b53005f7ec594468a2676545ee51071a6c41ee92bac603</originalsourceid><addsrcrecordid>eNp1kU9r3DAQxUVISTbbfoBcgiGX5uB2ZOuPfVy2bVJY2ktyFlrtOKvFtjYaO9BvXxknIRR60iD95s3TPMYuOXzhAPorcQkccuA6h7qqczhhCy7KVNS6PGULKAHyihfqnF0QHQBA1kKfsfOiKrioZblgt6vu2PrGOzv40GehybZtCLuMOrQx-_ZrlQ0hc6FvfOyynSfCzvd2wF229zSEY2upC-TpI_vQ2Jbw08u5ZA8_vt-v7_LN79uf69UmdwLUkNeF0FCViJVU2jmpldrK5FI2Gl0yJ1RlC6WVFBJRctDcKic4Yl1srVNQLtnNrLu3rTlG39n4xwTrzd1qY6Y7EACFlOKZJ_bzzB5jeBqRBtN5cti2tscwkuGVBiWkTItYsut_0EMYY59-Ynhamda1UJMgnykXA1HE5s0BBzMlYuZETErETImYyfDVi_K47XD31vEaQQKKGaD01D9ifDf6v6p_AVQrkvg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1947779461</pqid></control><display><type>article</type><title>Amplification of blood smear DNA to confirm disseminated histoplasmosis</title><source>Springer Nature</source><creator>Dieng, T. ; Massaly, A. ; Sow, D. ; Vellaissamy, S. ; Sylla, K. ; Tine, R. C. ; Dieng, Y. ; Hennequin, C.</creator><creatorcontrib>Dieng, T. ; Massaly, A. ; Sow, D. ; Vellaissamy, S. ; Sylla, K. ; Tine, R. C. ; Dieng, Y. ; Hennequin, C.</creatorcontrib><description>Background
The prevalence of the
Histoplasma capsulatum
var.
capsulatum
(
Hcc
) histoplasmosis may be underestimated West Africa, both because the diagnosis is not mentioned in the early stages of the disease and due to limited biological resources available.
Case Report
We report a case of disseminated histoplasmosis due to
Hcc
in a Senegalese HIV patient. The diagnosis was suspected following the demonstration of small encapsulated yeasts within neutrophils on a thin blood smear. It was further confirmed using a specific real-time PCR applied on a DNA specimen extracted from the thin blood smear.
Conclusion
To the best of our knowledge, this is the first case of
Hcc
infection diagnosed in Senegal. Blood smear may be a valuable screening tool in the case of bloodstream dissemination and can be used for further molecular approaches to confirm the diagnosis.</description><identifier>ISSN: 0300-8126</identifier><identifier>EISSN: 1439-0973</identifier><identifier>DOI: 10.1007/s15010-017-0989-0</identifier><identifier>PMID: 28214953</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Blood ; Case Report ; Case reports ; Deoxyribonucleic acid ; Diagnosis ; Disease control ; DNA ; DNA, Fungal - analysis ; Family Medicine ; General Practice ; Histoplasma - genetics ; Histoplasma - isolation & purification ; Histoplasmosis ; Histoplasmosis - blood ; Histoplasmosis - diagnosis ; Histoplasmosis - microbiology ; HIV ; HIV Infections ; Human immunodeficiency virus ; Humans ; Infectious Diseases ; Internal Medicine ; Leukocytes (neutrophilic) ; Life Sciences ; Male ; Medical diagnosis ; Medicine ; Medicine & Public Health ; Real-Time Polymerase Chain Reaction ; Senegal ; Smear ; Yeasts</subject><ispartof>Infection, 2017-10, Vol.45 (5), p.687-690</ispartof><rights>Springer-Verlag Berlin Heidelberg 2017</rights><rights>Infection is a copyright of Springer, 2017.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-9247083ee8567cc5766b53005f7ec594468a2676545ee51071a6c41ee92bac603</citedby><cites>FETCH-LOGICAL-c406t-9247083ee8567cc5766b53005f7ec594468a2676545ee51071a6c41ee92bac603</cites><orcidid>0000-0002-4528-927X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28214953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04002554$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Dieng, T.</creatorcontrib><creatorcontrib>Massaly, A.</creatorcontrib><creatorcontrib>Sow, D.</creatorcontrib><creatorcontrib>Vellaissamy, S.</creatorcontrib><creatorcontrib>Sylla, K.</creatorcontrib><creatorcontrib>Tine, R. C.</creatorcontrib><creatorcontrib>Dieng, Y.</creatorcontrib><creatorcontrib>Hennequin, C.</creatorcontrib><title>Amplification of blood smear DNA to confirm disseminated histoplasmosis</title><title>Infection</title><addtitle>Infection</addtitle><addtitle>Infection</addtitle><description>Background
The prevalence of the
Histoplasma capsulatum
var.
capsulatum
(
Hcc
) histoplasmosis may be underestimated West Africa, both because the diagnosis is not mentioned in the early stages of the disease and due to limited biological resources available.
Case Report
We report a case of disseminated histoplasmosis due to
Hcc
in a Senegalese HIV patient. The diagnosis was suspected following the demonstration of small encapsulated yeasts within neutrophils on a thin blood smear. It was further confirmed using a specific real-time PCR applied on a DNA specimen extracted from the thin blood smear.
Conclusion
To the best of our knowledge, this is the first case of
Hcc
infection diagnosed in Senegal. Blood smear may be a valuable screening tool in the case of bloodstream dissemination and can be used for further molecular approaches to confirm the diagnosis.</description><subject>Adult</subject><subject>Blood</subject><subject>Case Report</subject><subject>Case reports</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Disease control</subject><subject>DNA</subject><subject>DNA, Fungal - analysis</subject><subject>Family Medicine</subject><subject>General Practice</subject><subject>Histoplasma - genetics</subject><subject>Histoplasma - isolation & purification</subject><subject>Histoplasmosis</subject><subject>Histoplasmosis - blood</subject><subject>Histoplasmosis - diagnosis</subject><subject>Histoplasmosis - microbiology</subject><subject>HIV</subject><subject>HIV Infections</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Infectious Diseases</subject><subject>Internal Medicine</subject><subject>Leukocytes (neutrophilic)</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medical diagnosis</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Senegal</subject><subject>Smear</subject><subject>Yeasts</subject><issn>0300-8126</issn><issn>1439-0973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1kU9r3DAQxUVISTbbfoBcgiGX5uB2ZOuPfVy2bVJY2ktyFlrtOKvFtjYaO9BvXxknIRR60iD95s3TPMYuOXzhAPorcQkccuA6h7qqczhhCy7KVNS6PGULKAHyihfqnF0QHQBA1kKfsfOiKrioZblgt6vu2PrGOzv40GehybZtCLuMOrQx-_ZrlQ0hc6FvfOyynSfCzvd2wF229zSEY2upC-TpI_vQ2Jbw08u5ZA8_vt-v7_LN79uf69UmdwLUkNeF0FCViJVU2jmpldrK5FI2Gl0yJ1RlC6WVFBJRctDcKic4Yl1srVNQLtnNrLu3rTlG39n4xwTrzd1qY6Y7EACFlOKZJ_bzzB5jeBqRBtN5cti2tscwkuGVBiWkTItYsut_0EMYY59-Ynhamda1UJMgnykXA1HE5s0BBzMlYuZETErETImYyfDVi_K47XD31vEaQQKKGaD01D9ifDf6v6p_AVQrkvg</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Dieng, T.</creator><creator>Massaly, A.</creator><creator>Sow, D.</creator><creator>Vellaissamy, S.</creator><creator>Sylla, K.</creator><creator>Tine, R. C.</creator><creator>Dieng, Y.</creator><creator>Hennequin, C.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-4528-927X</orcidid></search><sort><creationdate>20171001</creationdate><title>Amplification of blood smear DNA to confirm disseminated histoplasmosis</title><author>Dieng, T. ; Massaly, A. ; Sow, D. ; Vellaissamy, S. ; Sylla, K. ; Tine, R. C. ; Dieng, Y. ; Hennequin, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-9247083ee8567cc5766b53005f7ec594468a2676545ee51071a6c41ee92bac603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Blood</topic><topic>Case Report</topic><topic>Case reports</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>Disease control</topic><topic>DNA</topic><topic>DNA, Fungal - analysis</topic><topic>Family Medicine</topic><topic>General Practice</topic><topic>Histoplasma - genetics</topic><topic>Histoplasma - isolation & purification</topic><topic>Histoplasmosis</topic><topic>Histoplasmosis - blood</topic><topic>Histoplasmosis - diagnosis</topic><topic>Histoplasmosis - microbiology</topic><topic>HIV</topic><topic>HIV Infections</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Infectious Diseases</topic><topic>Internal Medicine</topic><topic>Leukocytes (neutrophilic)</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medical diagnosis</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Senegal</topic><topic>Smear</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dieng, T.</creatorcontrib><creatorcontrib>Massaly, A.</creatorcontrib><creatorcontrib>Sow, D.</creatorcontrib><creatorcontrib>Vellaissamy, S.</creatorcontrib><creatorcontrib>Sylla, K.</creatorcontrib><creatorcontrib>Tine, R. C.</creatorcontrib><creatorcontrib>Dieng, Y.</creatorcontrib><creatorcontrib>Hennequin, C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dieng, T.</au><au>Massaly, A.</au><au>Sow, D.</au><au>Vellaissamy, S.</au><au>Sylla, K.</au><au>Tine, R. C.</au><au>Dieng, Y.</au><au>Hennequin, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amplification of blood smear DNA to confirm disseminated histoplasmosis</atitle><jtitle>Infection</jtitle><stitle>Infection</stitle><addtitle>Infection</addtitle><date>2017-10-01</date><risdate>2017</risdate><volume>45</volume><issue>5</issue><spage>687</spage><epage>690</epage><pages>687-690</pages><issn>0300-8126</issn><eissn>1439-0973</eissn><abstract>Background
The prevalence of the
Histoplasma capsulatum
var.
capsulatum
(
Hcc
) histoplasmosis may be underestimated West Africa, both because the diagnosis is not mentioned in the early stages of the disease and due to limited biological resources available.
Case Report
We report a case of disseminated histoplasmosis due to
Hcc
in a Senegalese HIV patient. The diagnosis was suspected following the demonstration of small encapsulated yeasts within neutrophils on a thin blood smear. It was further confirmed using a specific real-time PCR applied on a DNA specimen extracted from the thin blood smear.
Conclusion
To the best of our knowledge, this is the first case of
Hcc
infection diagnosed in Senegal. Blood smear may be a valuable screening tool in the case of bloodstream dissemination and can be used for further molecular approaches to confirm the diagnosis.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28214953</pmid><doi>10.1007/s15010-017-0989-0</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-4528-927X</orcidid></addata></record> |
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| ispartof | Infection, 2017-10, Vol.45 (5), p.687-690 |
| issn | 0300-8126 1439-0973 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_04002554v1 |
| source | Springer Nature |
| subjects | Adult Blood Case Report Case reports Deoxyribonucleic acid Diagnosis Disease control DNA DNA, Fungal - analysis Family Medicine General Practice Histoplasma - genetics Histoplasma - isolation & purification Histoplasmosis Histoplasmosis - blood Histoplasmosis - diagnosis Histoplasmosis - microbiology HIV HIV Infections Human immunodeficiency virus Humans Infectious Diseases Internal Medicine Leukocytes (neutrophilic) Life Sciences Male Medical diagnosis Medicine Medicine & Public Health Real-Time Polymerase Chain Reaction Senegal Smear Yeasts |
| title | Amplification of blood smear DNA to confirm disseminated histoplasmosis |
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