Inter‐laboratory assessment of flow cytometric monocyte HLA‐DR expression in clinical samples

Background: Diminished expression of human leukocyte antigen DR on circulating monocytes (mHLA‐DR) is a reliable indicator of immunosuppression in critically ill patients, predictive of both adverse outcome and septic complications. The objective of the present work was to test, in an inter‐laborato...

Full description

Saved in:
Bibliographic Details
Published in:Cytometry. Part B, Clinical cytometry Clinical cytometry, 2013-01, Vol.84B (1), p.59-62
Main Authors: Demaret, Julie, Walencik, Alexandre, Jacob, Marie‐Christine, Timsit, Jean‐François, Venet, Fabienne, Lepape, Alain, Monneret, Guillaume
Format: Article
Language:English
Subjects:
Flow Cytometry
HLA-DR Antigens - analysis
HLA-DR Antigens - immunology
HLA‐DR
Humans
Immune Tolerance
Life Sciences
monocyte
Monocytes - cytology
Monocytes - immunology
sepsis
Shock, Septic - immunology
standardization
Treatment Outcome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Diminished expression of human leukocyte antigen DR on circulating monocytes (mHLA‐DR) is a reliable indicator of immunosuppression in critically ill patients, predictive of both adverse outcome and septic complications. The objective of the present work was to test, in an inter‐laboratory clinical study, a standardized protocol for mHLA‐DR measurement by flow cytometry. Methods: mHLA‐DR was assessed in fresh whole blood according to a standardized staining protocol. Cells were analyzed on different flow cytometers (FC500, Navios, FACS Canto II) in different laboratories (Lyon and Grenoble). Results were expressed as numbers of antibodies bound per cell (AB/C). Results: Correlations between results were excellent (Pearson and interclass correlation coefficients > 0.98). Coefficients of variations for intra‐assay precision ranged from 1.9 to 3.2%. Conclusion: The present report highlights the robustness of this standardized flow cytometric protocol for mHLA‐DR measurement in multicentric clinical studies. © 2012 International Clinical Cytometry Society
ISSN:1552-4949
1552-4957
DOI:10.1002/cyto.b.21043