The human SRY transcript

SRY encodes the Y-linked testis-determining factor in humans. A predominant 900 bp transcript originates from a single exon and encompasses the putative SRY coding sequence. We show that in human adult testis SRY transcription involves multiple start sites. In addition to a previously defined major...

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Bibliographic Details
Published in:Human molecular genetics 1993-12, Vol.2 (12), p.2007-2012
Main Authors: Clepet, Christian, Schater, Alan J., Sinclair, Andrew H., Palmer, Mark S., Lovell-Badge, Robin, Goodfellow, Peter N.
Format: Article
Language:English
Subjects:
Adult
Base Sequence
Biological and medical sciences
Cell Line
DNA Primers
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Exons
Fundamental and applied biological sciences. Psychology
Gene Expression
Gene Library
genes
Humans
Hypoxanthine Phosphoribosyltransferase - genetics
Kruppel-Like Transcription Factors
Life Sciences
Male
man
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Polymerase Chain Reaction
sex determination
SRY gene
Testis - metabolism
tissues
Transcription Factors - genetics
Transcription, Genetic
Tumor Cells, Cultured
Y Chromosome
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Summary:SRY encodes the Y-linked testis-determining factor in humans. A predominant 900 bp transcript originates from a single exon and encompasses the putative SRY coding sequence. We show that in human adult testis SRY transcription involves multiple start sites. In addition to a previously defined major initiation site, transcripts originating at least 410 bp upstream of this site were detected. Using a cDNA specific RT - PCR assay, embryonic and adult human tissues were screened for SRY expression. In humans, SRY transcription is not restricted to the presumptive and the mature gonadal tissues In the embryo and the adult respectively but can be detected In a range of other locations. Two human cell lines, NTERA-2 cl.D1 (NT2/D1) and Hep G2, have been Identified which express SRY at similar levels to adult testis. The NT2/D1 SRY transcripts appear to have the same structure as those in adult testis. HMBA-Induced differentiation of NT2/D1 cells results in a diminution of SRY mRNA, while transcription of SRY In retinoic acid differentiated NT2/D1 Is unaffected.
ISSN:0964-6906
1460-2083
DOI:10.1093/hmg/2.12.2007