Confocal Raman data analysis of tufts and spindles at the human dentin-enamel junction

The aim of this article is to analyze the chemical mapping of tufts and spindles of the human dental enamel using confocal Raman microscopy measuring length, structuration and composition of spindles and tufts. we used Raman diffusion, based on the interaction between photons and optic phonons, to r...

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Bibliographic Details
Published in:Archives of oral biology 2021-11, Vol.131, p.105262-105262, Article 105262
Main Authors: Desoutter, Alban, Slimani, Amel, Tassery, Hervé, Cuisinier, Frédéric, Sauro, Salavatore, Salehi, Hamideh, Panayotov, Ivan
Format: Article
Language:English
Subjects:
confocal Raman microscopy
dentin
dentin enamel junction
Dentistry
Development Biology
enamel
Life Sciences
mineralized tissues
Morphogenesis
spindles
tuft
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Summary:The aim of this article is to analyze the chemical mapping of tufts and spindles of the human dental enamel using confocal Raman microscopy measuring length, structuration and composition of spindles and tufts. we used Raman diffusion, based on the interaction between photons and optic phonons, to reveal chemical bound. Adult molars were selected and longitudinally sectioned. Areas of 120 * 120 μm were scanned near the dentin-enamel junction and grooves. Spectra were collected and phosphate and proteins peak intensities images were reconstructed, related to HPA concentration. Images of Phosphate (PO43-, 960 cm−1) and protein (CH, 2800/3000 cm−1) intensities have been reconstructed. K-mean cluster has been calculated to compare centroid spectra from enamel, dentin and tuft or spindle. intensity profile revealed spindles as less mineralized areas than enamel, from 5 to 10 µm large. In the groove of molar, long tufts were found, more than 150 µm. Confocal Raman microscopy is a very interesting tool to characterize chemically secondary structure of enamel. The size of a tuft in the groove allows us make the hypothesis that they could play a role in long term resilience of mechanical stress. •Tufts and spindles are structural formations in enamel at the dentin-enamel junction.•The functions of tufts and spindles are unknown.•Chemical mapping Raman based shows tuft and spindle as hypomineralized structures.•Tuft localized in groove could play a mechanical role in resilience of teeth.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2021.105262