Small volume excitation and enhancement of dye fluorescence on a 2D photonic crystal surface

We demonstrate an easy-to-implement scheme for fluorescence enhancement and observation volume reduction using photonic crystals (PhCs) as substrates for microscopy. By normal incidence coupling to slow 2D-PhC guided modes, a 65 fold enhancement in the excitation is achieved in the near field region...

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Bibliographic Details
Published in:Optics express 2010-02, Vol.18 (4), p.3693-3699
Main Authors: Estrada, L C, Martinez, O E, Brunstein, M, Bouchoule, S, Le-Gratiet, L, Talneau, A, Sagnes, I, Monnier, P, Levenson, J A, Yacomotti, A M
Format: Article
Language:English
Subjects:
Contrast Media - analysis
Contrast Media - chemistry
Crystallization - methods
Fluorescent Dyes - analysis
Fluorescent Dyes - chemistry
Image Enhancement - methods
Microscopy, Fluorescence - methods
Optics
Physics
Surface Properties
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Summary:We demonstrate an easy-to-implement scheme for fluorescence enhancement and observation volume reduction using photonic crystals (PhCs) as substrates for microscopy. By normal incidence coupling to slow 2D-PhC guided modes, a 65 fold enhancement in the excitation is achieved in the near field region (100 nm deep and 1 microm wide) of the resonant mode. Such large enhancement together with the high spatial resolution makes this device an excellent substrate for fluorescence microscopies.
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.18.003693