Combining μX-ray fluorescence, μXANES and μXRD to shed light on Zn2+ cations in cartilage and meniscus calcifications

We aimedtoexaminethepresenceofZn,atraceelement,inosteoarthritis(OA)cartilageandmeniscusfrom patientsundergoingtotalkneejointreplacementforprimaryOA.WemappedCa2+ and Zn2+ at themesoscopic scalebyX-rayfluorescencemicroanalysis(X-ray) todeterminethespatialdistributionofthe 2elementsincartilage,X-ray ab...

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Published in:Journal of trace elements in medicine and biology 2013-10, Vol.27 (4), p.326-333
Main Authors: Dessombz, Arnaud, Nguyen, Christelle, Ea, Hang-Korng, Rouzière, Stephan, Foy, Eddy, Hannouche, Didier, Réguer, Solene, Picca, Frederic-Emmanuel, Thiaudière, Dominique, Lioté, Frédéric, Daudon, Michel, Bazin, Dominique
Format: Article
Language:English
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Life Sciences
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Summary:We aimedtoexaminethepresenceofZn,atraceelement,inosteoarthritis(OA)cartilageandmeniscusfrom patientsundergoingtotalkneejointreplacementforprimaryOA.WemappedCa2+ and Zn2+ at themesoscopic scalebyX-rayfluorescencemicroanalysis(X-ray) todeterminethespatialdistributionofthe 2elementsincartilage,X-ray absorptionnearedgestructurespectroscopytoidentifytheZnspecies,and X-ray diffractiontodeterminethechemicalnatureofthecalcification.Fouriertransforminfraredspectroscopy wasusedtodeterminethechemicalcompositionofcartilageandmeniscus.Ca2+ showeda heterogeneousspatialdistributioncorrespondingtothecalcificationswithincartilage(ormeniscus)or attheirsurface.Atleast2Zn2+ species werepresent:thefirstmaycorrespondtoZnembeddedinprotein (differentZnmetalloproteinsareknowntopreventcalcificationinbiologicaltissues),andthesecond maybeassociatedwithaZntrapinoratthesurfaceofthecalcification.CalcificationpresentinOA cartilagemaysignificantlymodifythespatialdistributionofZn;partoftheZnmaybetrappedinthecalcification andmayaltertheassociatedbiologicalfunctionofZnmetalloproteins.
ISSN:0946-672X
1878-3252
DOI:10.1016/j.jtemb.2013.02.001