Identification of general stress genes in Enterococcus faecalis

The susceptibility and the acquisition of tolerance of E. faecalis ATCC 19433 to heat, ethanol, bile salts, NaCl, H 2O 2 and pH shifts were determined. During exposure to these environmental stresses, protein synthesis analysed by 2-D electrophoresis revealed 167 stress proteins. Six stress protein...

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Bibliographic Details
Published in:International Journal of Food Microbiology 2000-04, Vol.55 (1), p.87-91
Main Authors: Rince, Alain, Flahaut, Sigrid, Auffray, Yanick
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Bacterial Proteins - biosynthesis
Bacteriology
bile salts
Biological and medical sciences
Chaperonin 60 - genetics
DnaK
Enterococcus faecalis
Enterococcus faecalis - genetics
Environmental stress
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
General stress protein
GroEL
Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - genetics
Life Sciences
Microbiology
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Summary:The susceptibility and the acquisition of tolerance of E. faecalis ATCC 19433 to heat, ethanol, bile salts, NaCl, H 2O 2 and pH shifts were determined. During exposure to these environmental stresses, protein synthesis analysed by 2-D electrophoresis revealed 167 stress proteins. Six stress protein were found to be induced by at least six of the eight treatments and considered to be general stress proteins (Gsp). Western blotting identified two of these Gsp as DnaK and GroEL. Analysis of the four other Gsp revealed that at least two of them were not previously described.
ISSN:0168-1605
1879-3460
DOI:10.1016/S0168-1605(00)00180-X