Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae)

We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2006-01, Vol.21 (7), p.1355-1358
Main Authors: Lazerges, M., Perrot, H., Antoine, E., Defontaine, A., Compere, C.
Format: Article
Language:English
Subjects:
Alexandrium minutum
Animals
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Chemical Sciences
Dinoflagellida - genetics
Dinophyceae
DNA
DNA, Algal - analysis
DNA, Algal - genetics
Electrochemistry - instrumentation
Electrochemistry - methods
Environmental Sciences
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
In Situ Hybridization - instrumentation
In Situ Hybridization - methods
Methods. Procedures. Technologies
Microbalance
Oligonucleotide Array Sequence Analysis - instrumentation
Oligonucleotide Array Sequence Analysis - methods
Quartz
Transducers
Various methods and equipments
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Description
Summary:We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram scale, for 3 h and makes it possible to follow frequency change from immobilization of the probe to hybridization of the complementary DNA target. This setup constitutes a biosensor, which is sensitive and selective, and the hybridization ratio between hybridized complementary DNA and immobilized DNA probes is 47%.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2005.05.013