Topology of mRNA chain in isolated eukaryotic double-row polyribosomes

In the process of protein synthesis, the translating ribosomes of eukaryotic cells form polyribosomes that are found to be multiplex functional complexes possessing elements of ordered spatial organization. As revealed by a number of electron microscopy studies, the predominant visible configuration...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2013-05, Vol.78 (5), p.445-454
Main Authors: Afonina, Zh. A., Myasnikov, A. G., Khabibullina, N. F., Belorusova, A. Yu, Menetret, J. -F., Vasiliev, V. D., Klaholz, B. P., Shirokov, V. A., Spirin, A. S.
Format: Article
Language:English
Subjects:
Algebraic topology
Analysis
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Chemical properties
Eukaryota - chemistry
Eukaryota - genetics
Eukaryota - metabolism
Eukaryotes
Genetics
Humans
Life Sciences
Messenger RNA
Microbiology
Nucleic Acid Conformation
Polyribosomes - chemistry
Polyribosomes - genetics
Polyribosomes - metabolism
Protein synthesis
Ribonucleic acid
Ribosomes
RNA
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
Tomography
Topology
Triticum aestivum
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Summary:In the process of protein synthesis, the translating ribosomes of eukaryotic cells form polyribosomes that are found to be multiplex functional complexes possessing elements of ordered spatial organization. As revealed by a number of electron microscopy studies, the predominant visible configurations of the eukaryotic polyribosomes are circles (circular polyribosomes) and two-stranded formations (so-called double-row polyribosomes). The “long” (i.e. heavy loaded) polyribosomes are usually represented by double-row structures, which can be interpreted as either topologically circular (“col-lapsed rings”), or topologically linear (zigzags or helices). In the present work we have analyzed the mRNA path within the eukaryotic polyribosomes, isolated from a wheat germ cell-free translation system, by integrating two approaches: the visualization of mRNA ends in polyribosomes by marking them with gold nanoparticles (3′-end) and initiating 40S subunits (5′-end), as well as by the cryoelectron tomography. Examination of the location of the mRNA markers in polyribosomes and mutual orientation of ribosomes in them has shown that the double-row polyribosomes of the same sample can have both circular and linear arrangements of their mRNA.
ISSN:0006-2979
1608-3040
1608-3040
DOI:10.1134/S0006297913050027