Evaluation of Adhesion, Proliferation, and Functional Differentiation of Dermal Fibroblasts on Glycosaminoglycan-Coated Polysulfone Membranes

It may be hypothesized that wound healing will benefit from polymer membranes coated with extracellular matrix macromolecules. Here we describe the behavior of dermal fibroblasts on polysulfone (PSU) membranes, and PSU membranes covered with chitosan (Ch), chondroitin sulfate (CS), or hyaluronan, us...

Full description

Saved in:
Bibliographic Details
Published in:Tissue engineering. Part A 2008-10, Vol.14 (10), p.1687-1697
Main Authors: Attia, Joan, Legendre, Florence, Nguyen, Quang Trong, Baugé, Catherine, Boumediene, Karim, Pujol, Jean Pierre
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Adhesion
Cell adhesion & migration
Cell Differentiation
Cell Proliferation
Collagen Type II - metabolism
Extracellular matrix
Fibroblasts - cytology
Fibroblasts - metabolism
Fibroblasts - ultrastructure
Gene expression
Glycosaminoglycans - chemistry
Health aspects
Humans
Life Sciences
Membranes
Membranes, Artificial
Methods
Microscopy, Electron, Scanning
Physiological aspects
Polymers
Skin - cytology
Skin - metabolism
Skin - ultrastructure
Sulfones
Tissue Culture Techniques - instrumentation
Tissue Culture Techniques - methods
Tissue engineering
Wound healing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It may be hypothesized that wound healing will benefit from polymer membranes coated with extracellular matrix macromolecules. Here we describe the behavior of dermal fibroblasts on polysulfone (PSU) membranes, and PSU membranes covered with chitosan (Ch), chondroitin sulfate (CS), or hyaluronan, using an additional intermediary ionic charge modification with poly-(acrylonitrile-co-methallyl sulfonate) (AN69), which allows binding of the polysaccharidic macromolecules. Cell adhesion, proliferation, cell signaling, and collagen gene expression were investigated. Ch and CS were found unable to support cell adhesion and proliferation. In contrast, both PSU and hyaluronic acid–coated PSU membranes appeared as suitable materials to culture fibroblasts and support their matrix synthesis capacity. Moreover, they induce type III collagen expression in addition to type I, suggesting that they promote a fetal-like environment that could be beneficial for wound healing.
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2007.0057