Hot and cold waves decrease sperm production and bias sex ratio in the parasitoid wasp Cotesia typhae (Hymenoptera, Braconidae)

• Published in: Journal of insect physiology 2023-09, Vol.149, p.104553-104553, Article 104553
• Main Authors: Bressac, Christophe, El Sabrout, Ahmed, Kifouche, Fatma, Anne, Melissa, Capdevielle-Dulac, Claire, Mougel, Florence, Kaiser, Laure
• Format: Article
• Language: English
• Subjects: Animal biology; Bioclimatology; Biocontrol; Ecology, environment; Invertebrate Zoology; Life Sciences; Male fertility; Overwintering; Reproduction; Temperature stress
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2023.104553

[Display omitted] •Cotesia typhae is a promising biocontrol parasitoid of Sesamia nonagrioides.•Heat and cold at the pupal stage reduce sperm in males that are subfertile.•Females mated with temperature stressed males store fewer sperm in their spermathecae.•Sex ratio of females mated with stressed males are biased.•Summer and winter conditions are unfavourable to C. typhae optimal reproduction. Parasitoid wasps are haplodiploid, meaning that sperm stored by egg laying females are only used to produce daughters. Thus, the sex ratio of the offspring depends on the availability of sperm after mating. In these insects, males are sensitive to temperature at the pupal stage. This stress leads to subfertility due to a drastic reduction in the number of sperm produced and transferred to females. Experiments were conducted under controlled conditions on the parasitoid wasp Cotesia typhae (Hymenoptera, Braconidae), a natural enemy of the invading pest Sesamia nonagrioides (Lepidoptera, Noctuidae). At 25–27 °C, sperm production was measured for 7 days, and found to reach a plateau at the third day of adult life. It leads to a final amount around 25,000 sperm per male. A male can successfully inseminate at least 10 females, producing predominantly female offspring. Sperm production decreased significantly after 1 day of pupal exposure to heat at 34 or 36 °C and 7 days of cold at 0, 5 or 10 °C. This highlights that both cold and heat are stressful. After mating with one male treated at 10 or 34 °C, females store fewer sperm than the control, and produce fewer daughters. The sex ratio of the offspring is male biased when males experienced temperature stresses during development, like other parasitoid wasps. In the field, C. typhae populations would be affected by heat and cold, at least at the pupal stage. This lowers overwintering risk in case this biological agent was introduced in Europe. This risk is both economical, as companies seek to establish costly continuous production to sell beneficial insects, and ecological as the introduced population would not settle in the ecosystem. Lastly, the transport and storage of this insect of agronomic interest would need to consider temperature variations to ensure successful application.