Neurogenic differentiation of human dental pulp stem cells using different induction protocols

Objective An investigation on neuronal differentiation capacity of human dental pulp stem cells (DPSCs) was still lacking. In this study, two different neuronal induction protocols were investigated and compared. Methods The neuronal differentiation was induced using chemical or growth factor induct...

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Bibliographic Details
Published in:Oral diseases 2014-05, Vol.20 (4), p.352-358
Main Authors: Osathanon, T, Sawangmake, C, Nowwarote, N, Pavasant, P
Format: Article
Language:English
Subjects:
Cell Differentiation
Cell Differentiation - drug effects
Cells, Cultured
Cytological Techniques
Cytological Techniques - methods
Dental Pulp
Dental Pulp - cytology
dental pulp stem cells
Dentistry
GABA receptors
Humans
Life Sciences
Medical research
neuronal differentiation
Neurons
Neurons - cytology
Notch signaling
Protein expression
Stem Cells
Stem Cells - cytology
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Summary:Objective An investigation on neuronal differentiation capacity of human dental pulp stem cells (DPSCs) was still lacking. In this study, two different neuronal induction protocols were investigated and compared. Methods The neuronal differentiation was induced using chemical or growth factor induction protocol. The differentiation was confirmed by the neurogenic mRNA and protein expression using polymerase chain reaction and immunocytochemistry, respectively. Results Chemical‐induced neuronal differentiation protocol promoted morphological change and β3‐TUBULIN protein expression. Though, SOX2, SOX9, and β3‐TUBULIN mRNA levels were not different compared with the control, indicating a defective differentiation. For growth factor induction protocol, the cells were exhibited neurite‐like cellular process and positively stained with β3‐TUBULIN. In addition, the increase in intracellular calcium was noted upon NMDA stimulation, implying the neuronal function. A dramatic increased mRNA expression of neurogenic markers [SOX2, SOX9, β3‐TUBULIN, and gamma‐aminobutyric acid (GABA receptors)] was noted as compared to the control. In addition, a remarkable increased expression of Notch signaling target gene, HEY1, was observed in growth factor‐induced DPSCs derived neuronal‐like cells compared with the control. Conclusion These data indicate that growth factor induction method is a preferable protocol for neuronal differentiation by DPSCs.
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.12119