Increasing the π‑Expansive Ligands in Ruthenium(II) Polypyridyl Complexes: Synthesis, Characterization, and Biological Evaluation for Photodynamic Therapy Applications

• Published in: Inorganic chemistry 2023-11, Vol.62 (45), p.18510-18523
• Main Authors: Pozza, Maria Dalla, Mesdom, Pierre, Abdullrahman, Ahmad, Prieto Otoya, Tayler D., Arnoux, Philippe, Frochot, Céline, Niogret, Germain, Saubaméa, Bruno, Burckel, Pierre, Hall, James P., Hollenstein, Marcel, Cardin, Christine J., Gasser, Gilles
• Format: Article
• Language: English
• Subjects: Chemical Sciences; Medicinal Chemistry
• ISSN: 0020-1669; 1520-510X
• DOI: 10.1021/acs.inorgchem.3c02606

Lack of selectivity is one of the main issues with currently used chemotherapies, causing damage not only to altered cells but also to healthy cells. Over the last decades, photodynamic therapy (PDT) has increased as a promising therapeutic tool due to its potential to treat diseases like cancer or bacterial infections with a high spatiotemporal control. Ruthenium­(II) polypyridyl compounds are gaining attention for their application as photosensitizers (PSs) since they are generally nontoxic in dark conditions, while they show remarkable toxicity after light irradiation. In this work, four Ru­(II) polypyridyl compounds with sterically expansive ligands were studied as PDT agents. The Ru­(II) complexes were synthesized using an alternative route to those described in the literature, which resulted in an improvement of the synthesis yields. Solid-state structures of compounds [Ru­(DIP) 2 phen]­Cl 2 and [Ru­(dppz) 2 phen]­(PF 6 ) 2 have also been obtained. It is well-known that compound [Ru­(dppz)­(phen)2]­Cl2 binds to DNA by intercalation. Therefore, we used [Ru­(dppz) 2 phen]­Cl 2 as a model for DNA interaction studies, showing that it stabilized two different sequences of duplex DNA. Most of the synthesized Ru­(II) derivatives showed very promising singlet oxygen quantum yields, together with noteworthy photocytotoxic properties against two different cancer cell lines, with IC50 in the micro- or even nanomolar range (0.06–7 μM). Confocal microscopy studies showed that [Ru­(DIP) 2 phen]­Cl 2 and [Ru­(DIP) 2 TAP]­Cl 2 accumulate preferentially in mitochondria, while no mitochondrial internalization was observed for the other compounds. Although [Ru­(dppn) 2 phen]­(PF 6 ) 2 did not accumulate in mitochondria, it interestingly triggered an impairment in mitochondrial respiration after light irradiation. Among others, [Ru­(dppn) 2 phen]­(PF 6 ) 2 stands out for its very good IC50 values, correlated with a very high singlet oxygen quantum yield and mitochondrial respiration disruption.