Purification and Characterization of a NADPH-DependentAldehyde Reductase from Mung Bean That DetoxifiesEutypine, a Toxin from Eutypa lata1

Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde)is a toxin produced by Eutypa lata, the causal agent of eutypadieback in the grapevine (Vitis vinifera). Eutypine is enzymaticallyconverted by numerous plant tissues into eutypinol (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzyl alcohol),...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1999, Vol.1, p.621-626
Main Authors: Colrat, Ségolène, Latché, Alain, Guis, Monique, Pech, Jean-Claude, Bouzayen, Mondher, Fallot, Jean, Roustan, Jean-Paul
Format: Article
Language:English
Subjects:
Agricultural sciences
Agronomy
Biotechnology
Life Sciences
Vegetal Biology
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Summary:Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde)is a toxin produced by Eutypa lata, the causal agent of eutypadieback in the grapevine (Vitis vinifera). Eutypine is enzymaticallyconverted by numerous plant tissues into eutypinol (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzyl alcohol), a metabolite that isnontoxic to grapevine. We report a four-step procedure for thepurification to apparent electrophoretic homogeneity of a eutypinereducingenzyme (ERE) from etiolated mung bean (Vigna radiata)hypocotyls. The purified protein is a monomer of 36 kD, usesNADPH as a cofactor, and exhibits a Km value of 6.3 mM foreutypine and a high affinity for 3- and 4-nitro-benzaldehyde. Theenzyme failed to catalyze the reverse reaction using eutypinol as asubstrate. ERE detoxifies eutypine efficiently over a pH range from6.2 to 7.5. These data strongly suggest that ERE is an aldehydereductase that could probably be classified into the aldo-keto reductasesuperfamily. We discuss the possible role of this enzyme ineutypine detoxification.
ISSN:0032-0889
1532-2548